Anti-CNTD1 Rabbit Polyclonal Antibody for WB, ELISA - Q8N815

Core Product Specifications and Parameters

Biological Background: CNTD1 Function and Localization

• CNTD1 (Cyclin N-terminal domain-containing protein 1) is a meiosis-specific protein encoded by the CNTD1 gene, also known as COSA1, playing a critical role in crossover formation during meiotic recombination.
• It participates in the differentiation of crossover-specific recombination intermediates through direct interaction with PRR19, ensuring proper crossover designation.
• CNTD1 stimulates crossover formation by interacting with the replication factor C subunits RFC3 and RFC4, linking recombination to DNA metabolism.
• The protein also regulates cell-cycle progression via interaction with CDC34, promoting ubiquitination of WEE1 and thereby modulating the G2/M checkpoint.
• CNTD1 may be involved in an active deselection process that destabilizes or removes excess pre-crossover intermediates, maintaining crossover homeostasis.
• Subcellularly, CNTD1 localizes to the nucleus, cytoplasm, and chromosomes, consistent with its dynamic roles during meiotic prophase I.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-120) targets the N-terminal region of human CNTD1; consider that the antibody may recognize multiple isoforms arising from alternative splicing.
• For Western blotting, CNTD1 has a predicted molecular weight of ~37 kDa; use appropriate gel percentages and validate detection under reducing conditions in human testis or other meiosis-competent samples.
• In ELISA, the polyclonal nature allows use as either capture or detection reagent; proper titration and pairing with a compatible secondary antibody are recommended.
• Verify antibody specificity using a knockdown or knockout control where possible, given the potential for cross-reactivity with other cyclin-domain proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines procurement for diagnostic manufacturers and research labs by supplying validated IVD raw materials like this CNTD1 polyclonal antibody, which supports meiosis and fertility-related research. From optimized monoclonal/polyclonal antibody pairs and bulk ancillary reagents to rare target sourcing, CamelBio bridges the gap from concept to clinic.