Anti-CNR1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P21554

Core Product Specifications and Parameters

Biological Background: CNR1 Function and Localization

• CNR1 encodes cannabinoid receptor 1, a G protein-coupled receptor that binds endogenous cannabinoids (anandamide, 2-AG) and phytocannabinoids like THC. Related references: PMID:15620723, PMID:27768894, PMID:27851727
• It mediates diverse physiological effects including regulation of food intake, memory, gastrointestinal motility, catalepsy, anxiety, and pain, primarily via Gi/o-mediated inhibition of adenylate cyclase and reduction in cAMP. Related references: PMID:1718258, PMID:21895628, PMID:27768894
• In the brain, CNR1 is highly expressed in the hippocampus and hypothalamus, where it modulates synaptic plasticity and energy homeostasis.
• It inhibits voltage-gated calcium channels in vascular smooth muscle, contributing to vasodilation, and also regulates lipogenesis and energy expenditure in peripheral tissues.
• Activation in macrophages can trigger NLRP3 inflammasome-mediated IL-1β and IL-18 secretion, linking cannabinoid signaling to inflammation and type 2 diabetes. Related references: PMID:23955712
• Localization: cell membrane, membrane rafts, mitochondrial outer membrane, and presynaptic terminals; undergoes alternative splicing and post-translational modifications (palmitoylation, phosphorylation, glycosylation).
• Widely expressed with highest levels in fetal and adult brain; isoform 1 is predominant, while isoforms 2 and 3 show lower expression and distinct ligand responses.

Experimental Guidance and Technical Tips

• The immunogen covers the C-terminal 72 amino acids (residues 401–472), a region that may be conserved across species and accessible in both native and denaturing conditions, supporting use in WB and IHC-P.
• For Western blotting, the predicted molecular weight is approximately 53 kDa; post-translational modifications may cause slight shifts. Include appropriate positive controls such as brain tissue lysates.
• For IHC-P, optimize antigen retrieval and antibody dilution for your tissue and fixation method; known CNR1-rich regions (e.g., hippocampus) can serve as positive controls.
• As a polyclonal antibody, lot-to-lot variability may occur; verify consistency and consider re-validation for critical quantitative applications.
• For ELISA, this antibody may be used as capture or detection reagent; for sandwich ELISA, pairing with a matched monoclonal antibody is recommended for quantitative detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories comprehensive access to IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-CNR1 reagent, and bulk ancillary supplies. For neuroscience and metabolic research applications, we support rare-target raw-material sourcing and provide technical consultation from concept to clinic, ensuring a reliable supply chain for your assay development needs.