Anti-CNPY3 Rabbit Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q9BT09

Core Product Specifications and Parameters

Biological Background: CNPY3 Function and Localization

• Protein canopy homolog 3 (CNPY3), also known as PRAT4A, TNRC5, ERDA5, CTG4A, is a member of the canopy family, acting as a co-chaperone for HSP90B1 specifically for Toll-like receptors (TLRs) except TLR3, facilitating their proper folding and exit from the endoplasmic reticulum (ER).
• It plays a critical role in both innate and adaptive immune responses by controlling the trafficking of TLRs to the cell surface, thereby enabling pathogen recognition and downstream signaling.
• CNPY3 is localized in the endoplasmic reticulum and is a glycoprotein with a signal peptide, highlighting its involvement in the secretory pathway.
• The protein contains disulfide bonds and a coiled-coil domain, contributing to its chaperone function and interactions with client proteins.
• Alternative splicing yields multiple isoforms, and mutations in this gene are associated with epileptic encephalopathy, indicating neurological relevance.
• Post-translational modifications include glycosylation, which is consistent with its ER localization and trafficking roles.
• Keywords: chaperone, innate immunity, epilepsy disease variant, suggesting a link between immune function and neuronal stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 31-278, encompassing the mature protein region without the signal peptide, which likely enhances specificity for the processed, ER-localized form.
• For Western blot (WB), expect a band around 31 kDa; consider the possibility of glycosylation shifting the apparent molecular weight.
• In immunohistochemistry (IHC-P), validate with appropriate tissue controls expressing CNPY3, such as immune-related tissues.
• For immunoprecipitation (IP), the polyclonal nature may allow efficient capture of native CNPY3 complexes; verify with known interaction partners like HSP90B1.
• ELISA can be used for quantitative analysis; ensure coating conditions preserve the protein's conformation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials. Our CNPY3 rabbit polyclonal antibody is part of a comprehensive portfolio for immune signaling and endoplasmic reticulum chaperone studies. We support your projects from concept to clinic with validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare target raw materials.