Anti-CNPase Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P09543

Core Product Specifications and Parameters

Biological Background: CNPase Function and Localization

• CNPase is a myelin-associated enzyme that catalyzes the hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides, playing a crucial role in myelin structure and function.
• Beyond its enzymatic activity, CNPase regulates the mitochondrial permeability transition pore (mPTP) by localizing to mitochondria, thereby influencing both apoptotic and necrotic cell death pathways.
• CNPase exhibits antiviral properties: it suppresses SARS-CoV-2 virion assembly (PubMed:38117830) and inhibits HIV-1 virion assembly through direct interaction with the GAG protein. Related references: PMID:38117830
• The protein is localized to multiple subcellular compartments including the melanosome, mitochondrion, and cell membrane.
• Post-translational modifications include acetylation, methylation, and prenylation, which may modulate its function and localization.
• Mutations in the CNP gene are associated with leukodystrophy and neurodegeneration, highlighting its importance in neurological health.
• Multiple isoforms exist due to alternative splicing, contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 150–421 of human CNPase (C-terminal portion); thus, epitope recognition is likely directed to this region. Consider this when designing complementary reagents.
• For Western blot, a band around 48 kDa is expected, but alternative splicing may produce additional isoforms at slightly different sizes. Validate in your specific sample system.
• For immunofluorescence (IF-P), optimize fixation and permeabilization protocols to preserve membrane and mitochondrial localization signals.
• In ELISA applications, use appropriate coating and blocking conditions, and include positive and negative controls to ensure specificity, especially when testing across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated sourcing partner for diagnostic manufacturers and research institutions, offering a broad catalog of IVD raw materials, including this anti-CNPase polyclonal antibody validated for neuroscience and antiviral research. Beyond primary antibodies, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assist in rare-target raw-material sourcing to support your entire assay development workflow. Rely on CamelBio for dependable raw materials from concept to clinical application.