Anti-CNPase Rabbit Monoclonal Antibody for WB, IF-P, IHC-P, ELISA - P09543

Core Product Specifications and Parameters

Biological Background: CNPase Function and Localization

• CNPase (2',3'-cyclic-nucleotide 3'-phosphodiesterase), encoded by the CNP gene, is also known as CNP1 and is associated with the disorder HLD20.
• It catalyzes the hydrolysis of 2',3'-cyclic nucleotides to 2'-nucleotides, a reaction critical for myelin maintenance and integrity in the nervous system.
• In mitochondria, CNPase regulates the mitochondrial permeability transition pore (mPTP), thereby participating in the control of both apoptotic and necrotic cell death pathways.
• CNPase acts as an antiviral factor by directly interacting with viral GAG proteins to suppress virion assembly, as demonstrated for both SARS-CoV-2 and HIV-1. Related references: PMID:38117830
• The protein localizes to melanosomes, mitochondria, and the cell membrane, reflecting its multilayered cellular roles.
• Post-translational modifications include acetylation, methylation, prenylation, and phosphorylation; the protein also possesses RNA-binding activity and is classified as a lipoprotein.
• CNPase is a reference proteome member with a 3D structure determined by direct protein sequencing, and its expression is alternatively spliced.

Experimental Guidance and Technical Tips

• The immunogen is a C-terminal peptide (aa 322–421); consider verifying reactivity in systems expressing both full-length and truncated CNPase isoforms.
• For WB, a band near 48kDa is expected, but post-translational modifications or alternative splicing may produce additional bands—validate using appropriate controls (e.g., brain lysate).
• For IF-P and IHC-P, permeabilization is essential to detect mitochondrial and membrane-localized CNPase; optimize fixation and antigen retrieval steps.
• The antibody is reported to cross-react with mouse and rat; when using these species, confirm specificity in the target tissue (e.g., CNS samples).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your integrated sourcing partner for high-quality IVD raw materials, including validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. Our anti-CNPase antibody supports research into myelination disorders, neurodegeneration, and antiviral mechanisms. We also specialize in rare target custom antibody development to meet your unique assay needs.