Anti-CNPase Polyclonal Antibody for WB, IHC-P, ELISA - P09543

Core Product Specifications and Parameters

Biological Background: CNPase Function and Localization

• CNPase is a myelin-associated enzyme that hydrolyzes 2',3'-cyclic nucleotides to 2'-nucleotides, playing a role in oligodendrocyte function and myelin maintenance.
• In mitochondria, CNPase regulates the mitochondrial permeability transition pore (mPTP), thereby participating in apoptotic and necrotic cell death pathways.
• Acts as an antiviral factor by suppressing SARS-CoV-2 virion assembly. Related references: PMID:38117830
• Also inhibits HIV-1 virion assembly via direct interaction with the GAG protein.
• Localizes to melanosomes, mitochondria, and the cell membrane, indicating diverse subcellular functions.
• Post-translational modifications include acetylation, phosphorylation, and prenylation; alternatively spliced isoforms exist.
• Associated with leukodystrophy and neurodegeneration, underscoring its clinical importance.
• Molecular weight ~48 kDa (47579 Da).

Experimental Guidance and Technical Tips

• The immunogen spans a large portion (aa 152-421) of CNPase, covering both major splicing isoforms; perform validation in brain tissue lysates or purified myelin preparations.
• For Western blot, expect bands near 48 kDa; consider optimizing antibody dilution using relevant positive control lysates (e.g., human, mouse, or rat brain).
• In IHC-P, antigen retrieval may improve signal; test on paraffin-embedded brain sections with appropriate positive and negative controls.
• ELISA sensitivity and specificity should be confirmed using recombinant CNPase protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined procurement channel for IVD raw materials in neurobiology and infectious disease. Our catalog includes validated anti-CNPase polyclonal antibodies, matched recombinant antigens, and ancillary reagents—all available in bulk quantities. For custom or rare target sourcing, our technical team provides consulting to ensure seamless integration from concept to clinical application.