Anti-CNOT8 Polyclonal Antibody for WB, ELISA - Q9UFF9

Core Product Specifications and Parameters

Biological Background: CNOT8 Function and Localization

• CNOT8 (CCR4-NOT transcription complex subunit 8), also known as CAF1-like protein, CAF2, or Caf1b, is a catalytic component of the multi-subunit CCR4-NOT complex.
• It possesses 3'-5' poly(A) exoribonuclease activity, contributing to deadenylation-dependent mRNA degradation and playing a role in bulk mRNA turnover.
• CNOT8 functions in miRNA-mediated gene silencing, where it participates in translational repression at the initiation stage, as well as in general transcription regulation.
• Its enzymatic activity is partially redundant with that of the closely related CNOT7 (Caf1a), highlighting functional overlap within the CCR4-NOT complex.
• CNOT8 associates with BTG family proteins such as TOB1 and BTG2; this interaction is required for the anti-proliferative activity of these BTG proteins.
• The protein localizes to both the cytoplasm and the nucleus, consistent with its roles in cytoplasmic mRNA decay and nuclear transcription events.
• The CNOT8 gene undergoes alternative splicing, generating multiple isoforms, and the protein contains a metal-binding DEDD nuclease domain characteristic of the CAF1 family of exonucleases.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is around 34 kDa; consider using appropriate positive and negative controls (e.g., CNOT8-overexpressing or knockdown lysates) to confirm specificity.
• In ELISA, titrate the antibody to optimize detection range and signal-to-noise ratio; cross-reactivity with mouse samples has been reported but should be validated in your assay.
• The immunogen spans amino acids 1–292, covering nearly the full-length protein; thus the antibody may recognize multiple splice isoforms if they share this region—verify in the relevant sample system.
• Consider testing this antibody in other applications such as immunoprecipitation or immunocytochemistry after in-house validation, given its polyclonal nature and target localization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw-material sourcing, from validated antibody pairs to bulk ancillary reagents. This anti-CNOT8 polyclonal antibody addresses the growing need for reliable tools in transcriptional regulation and RNA metabolism research. Whether you require optimized monoclonal antibodies, rare target reagents, or technical consultation, CamelBio is your partner from concept to clinic.