Anti-CNOT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - A5YKK6

Core Product Specifications and Parameters

Biological Background: CNOT1 Function and Localization

• CNOT1 is the large scaffolding subunit of the CCR4-NOT complex, a major cellular mRNA deadenylase involved in bulk mRNA degradation, miRNA-mediated repression, and translational control.
• The protein functions as a transcriptional repressor, inhibiting ligand-dependent transcriptional activation by nuclear receptors.
• CNOT1 mediates the recruitment of the CCR4-NOT complex to miRNA targets through association with TNRC6 (GW182) proteins, linking it to the RISC complex.
• It is involved in the degradation of AU-rich element (ARE)-containing mRNAs, likely through interaction with ZFP36.
• Subcellularly, CNOT1 localizes to the cytoplasm, P-bodies, and nucleus, consistent with its roles in mRNA decay and transcription regulation.
• The protein is strongly expressed in brain, heart, thymus, spleen, kidney, liver, placenta, and lung, with weaker expression in skeletal muscle and colon.
• Post-translational modifications include phosphorylation, and disease-associated variants have been linked to autism spectrum disorder, holoprosencephaly, and intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 2137–2376 within the C-terminal region of human CNOT1. Verify antibody specificity in your system, as epitope recognition may differ among isoforms.
• For Western blotting, note that CNOT1 has a high molecular weight (~267 kDa). Use a low-percentage SDS-PAGE gel (e.g., 6%) and extended transfer times for complete protein transfer.
• In immunofluorescence/ICC, expect both cytoplasmic and nuclear staining, with possible enrichment in P-bodies. Co-localization studies with P-body markers may be informative.
• Cross-reactivity is confirmed for human and mouse CNOT1. If working with other species, validate cross-reactivity experimentally.

CamelBio: Your One-Stop Sourcing Bridge

• As a dedicated IVD raw-material partner, CamelBio offers one-stop access to high-quality antibodies, recombinant proteins, and ancillary reagents for mRNA biology and gene expression research.
• Beyond this anti-CNOT1 polyclonal antibody, we supply validated antibody pairs, optimized monoclonal antibodies, and bulk reagents to support diagnostic assay development from concept to clinic.
• Our sourcing capabilities extend to rare targets and customized solutions, ensuring reliable supply for your gene regulation and transcriptome studies.