Anti-CNNM1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NRU3

Core Product Specifications and Parameters

Biological Background: CNNM1 Function and Localization

• Metal transporter CNNM1 (also designated ancient conserved domain-containing protein 1, Cyclin-M1, ACDP1, or CLP-1) is a probable metal transporter involved in ion homeostasis.
• It is predicted to mediate transport of metal ions across the cell membrane, based on its structural features and functional classification.
• The protein contains CBS (cystathionine beta-synthase) domain repeats and multiple transmembrane helices, which are characteristic of the CNNM transporter family.
• CNNM1 localizes to the cell membrane as a multi-pass membrane protein, consistent with its role in transmembrane ion transport.
• Tissue expression is tightly restricted to the brain and testis, suggesting a specialized role in metal handling within these organs.
• Alternative splicing generates multiple isoforms, and the protein is subject to phosphorylation, as indicated by phosphoproteomic studies.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 104 kDa. Ensure adequate protein extraction and transfer conditions for this high-molecular-weight membrane protein.
• In immunofluorescence/ICC, permeabilization may be required to access the intracellular epitopes of this transmembrane protein. Validate staining in known expression-positive tissues (brain, testis).
• For ELISA, consider using the recombinant immunogen or a validated standard for quantitative assays; polyclonal antibodies may require careful titration to optimize signal-to-noise ratio.
• Since CNNM1 expression is limited to brain and testis, select appropriate positive control samples for all applications to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material partner, connecting diagnostic manufacturers, laboratories, and research institutes with validated antibodies, bulk reagents, and rare-target sourcing solutions. For neuroscience and reproductive biology research, CamelBio can supply optimized polyclonal antibodies, matched antibody pairs, and ancillary reagents to streamline your assay development. Our one-stop platform supports your entire workflow, from discovery to clinical application, ensuring reliable access to high-quality raw materials.