Anti-CNN3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q15417

Core Product Specifications and Parameters

Biological Background: CNN3 (Calponin-3) Function and Localization

• CNN3 encodes calponin-3, also known as the acidic isoform of calponin, a thin filament-associated protein involved in regulating smooth muscle contraction.
• The protein directly binds actin, calmodulin, and tropomyosin, and its interaction with actin inhibits actomyosin Mg-ATPase activity, modulating contractility.
• Calponin-3 is expressed in both smooth muscle and non-smooth muscle tissues, indicating broader functional roles beyond contractile regulation.
• Alternative splicing of CNN3 generates multiple isoforms, contributing to functional diversity.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate protein activity and interactions.
• The protein contains repeat motifs (calponin-like repeats) characteristic of the calponin family, facilitating protein-protein interactions.
• The molecular weight on SDS-PAGE is ~36 kDa (theoretical 36.4 kDa).

Experimental Guidance and Technical Tips

• The immunogen was designed from a region within amino acids 220-329 of human CNN3; sequence alignment shows high conservation in mouse and rat, supporting the observed cross-reactivity.
• For Western blot (WB), a band around 36 kDa is expected; consider using whole-cell lysates from smooth muscle cell lines (e.g., human bronchial smooth muscle cells) or tissues.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimized fixation and permeabilization protocols may be required to detect filament-associated calponin-3; validate in the relevant sample system.
• For ELISA, the antibody can be used as a capture or detection reagent; appropriate antigen coating and antibody dilutions need empirical optimization.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a one-stop sourcing solution for IVD raw materials. This anti-CNN3 polyclonal antibody supports research into smooth muscle contraction and actin-binding mechanisms. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can assist in procuring rare target raw materials—streamlining your transition from concept to clinic.