Anti-CNGA4 Rabbit Polyclonal Antibody for WB, ELISA - Q8IV77

Core Product Specifications and Parameters

Biological Background: CNGA4 Function and Localization

• CNGA4 (Cyclic nucleotide-gated channel alpha-4) is a pore-forming subunit of the olfactory cyclic nucleotide-gated (CNG) channel, also known as OCNC2. It is encoded by the CNGA4 gene in humans.
• Primarily expressed in olfactory sensory neurons, CNGA4 operates in the cilia where it mediates the conversion of odorant chemical stimuli into electrical signals.
• Upon odorant stimulation, CNGA4 facilitates cAMP-dependent Ca²⁺ influx, triggering neuron depolarization and an increase in intracellular Ca²⁺ levels.
• The rise in Ca²⁺ both potentiates the olfactory response via activation of Ca²⁺-dependent Cl⁻ channels and serves as a negative feedback signal to desensitize the channel, enabling rapid adaptation to odorants.
• CNGA4 conducts both cAMP- and cGMP-gated ion currents with permeability to monovalent and divalent cations, playing a central role in olfactory signal transduction.
• Localized to the cilium membrane (a specialized cell projection), CNGA4 is a multi-pass transmembrane protein that belongs to the ligand-gated ion channel family.
• The protein contains cyclic nucleotide-binding domains and exhibits alternative splicing, suggesting potential isoform diversity.

Experimental Guidance and Technical Tips

• The immunogen is designed from the C-terminal region (amino acids 416-575) of human CNGA4, which may confer high specificity to the target.
• For Western blot (WB), the predicted molecular weight of CNGA4 is approximately 66 kDa. Validate detection in olfactory epithelial tissue or cell lysates expressing CNGA4.
• For ELISA applications, this polyclonal antibody can be tested as a capture or detection antibody. Optimize the antibody concentration and pair with a suitable secondary antibody.
• Cross-reactivity with mouse CNGA4 is reported; experimental validation in the relevant sample system is recommended to confirm specificity.
• Consider using appropriate controls, such as knockout cell lines or blocking peptides, to verify specific binding.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated one-stop sourcing partner for IVD raw materials, CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of antibodies and ancillary reagents. This anti-CNGA4 polyclonal antibody is designed for researchers investigating olfactory signal transduction and ion channel function, and CamelBio can further assist with validated antibody pairs, bulk reagent orders, and customized rare-target sourcing. From concept to clinic, we streamline your procurement process with high-quality, traceable products.