Anti-CNDP2 Rabbit Polyclonal Antibody for WB and ELISA - Q96KP4

Core Product Specifications and Parameters

Biological Background: CNDP2 Function and Localization

• CNDP2 (CNDP dipeptidase 2), also known as CN2, CPGL, PEPA, or HEL-S-13, is a member of the M20 metallopeptidase family and functions as a cytosolic non-specific dipeptidase.
• It catalyzes the hydrolysis of amide bonds in dipeptides, with broad substrate specificity including L-cysteinylglycine, a key intermediate in glutathione metabolism. Related references: PMID:12473676 PMID:19346245 PMID:25964343
• Under conditions of amino acid and lactate excess, CNDP2 mediates reverse proteolysis to produce N-lactoyl-amino acids such as N-[(S)-lactoyl]-L-phenylalanine, an exercise-induced metabolite regulating food intake and systemic energy balance. Related references: PMID:25964343
• The enzyme plays a role in cell cycle arrest and apoptosis, and its down-regulation in gastric cancer tissues suggests a tumor-suppressive function. Related references: PMID:17121880 PMID:24395568
• CNDP2 is ubiquitously expressed, with elevated protein levels in kidney and liver, and is also present in peripheral blood leukocytes and fetal tissues. Related references: PMID:12473676
• Subcellularly, CNDP2 localizes to the cytoplasm/cytosol, and it undergoes post-translational modifications including acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 210 amino acids of human CNDP2 (NP_060705.2), a domain that is well-conserved across species (human, mouse, rat) and likely to generate antibodies recognizing both native and denatured protein; for Western blot, expect a band near 53 kDa.
• Given CNDP2's ubiquitous expression, perform preliminary validation in lysates from human kidney or liver cell lines, and consider subcellular fractionation to enrich for cytosolic protein if signals are weak.
• For ELISA, use recombinant CNDP2 as a coating antigen and optimize antibody concentrations to minimize non-specific binding.
• As a polyclonal antibody, cross-reactivity with related dipeptidases is possible; confirm specificity by siRNA knockdown or blocking peptide competition in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner providing diagnostic manufacturers and researchers with one-stop access to IVD raw materials, technical services, and consulting—from concept to clinic. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for immunoassay development. The CNDP2 polyclonal antibody supports your research in cancer metabolism and glutathione metabolism, and we can also assist in sourcing rare-target raw materials to accelerate your project.