Anti-CNBP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P62633

Core Product Specifications and Parameters

Biological Background: CNBP Function and Localization

• CNBP (CCHC-type zinc finger nucleic acid binding protein), also known as cellular nucleic acid-binding protein or zinc finger protein 9, is a single-stranded DNA-binding protein that preferentially binds the sterol regulatory element (SRE) sequence 5'-GTGCGGTG-3', mediating transcriptional repression. Related references: PMID:2562787
• It also functions as a transactivator of the Myc promoter, linking it to cell proliferation control (By similarity).
• CNBP binds single-stranded RNA in a sequence-specific manner and specifically targets G-rich elements in target mRNA coding sequences, preventing G-quadruplex structure formation and thereby supporting translation. Related references: PMID:28329689
• The protein contains seven CCHC-type zinc finger domains that confer nucleic acid binding and is subject to post-translational modifications including acetylation, methylation, and phosphorylation, which may regulate its activity.
• Subcellularly, CNBP is found in the nucleus, cytoplasm, and endoplasmic reticulum, reflecting its diverse roles in gene regulation and RNA metabolism.
• It is expressed in multiple tissues, including liver, kidney, spleen, testis, lung, muscle, and adrenal glands, with alternative splicing generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 70–170 of human CNBP (NP_001120668.1). This region is conserved, and the antibody is reported to cross-react with mouse and rat; however, perform sequence alignment if using in other species.
• For Western blotting, a predominant band at ~19 kDa is expected. Additional bands may represent post-translationally modified forms or isoforms. Consider using appropriate blocking and detection conditions.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; optimize according to your tissue and protocol. Include positive and negative controls.
• For immunofluorescence (IF/ICC), verify the intracellular distribution agrees with known nuclear and cytoplasmic localization. Fixation and permeabilization conditions can affect staining.
• The antibody has been tested in ELISA; ensure coating conditions and detection format are validated in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a comprehensive portfolio of IVD raw materials, from validated primary antibodies like this anti-CNBP polyclonal to ancillary reagents and custom procurement services. Whether you are developing transcriptional regulation assays or studying nucleic acid-binding proteins, our one-stop sourcing model connects you with consistent, research-grade reagents and technical support to accelerate your project from discovery to clinical application. Leverage our supply network for bulk orders, matched antibody pairs, and hard-to-find targets.