Anti-CMTM6 Rabbit Monoclonal Antibody for WB, ELISA - Q9NX76

Core Product Specifications and Parameters

Biological Background: CMTM6 Function and Localization

• CMTM6 (CKLF-like MARVEL transmembrane domain-containing protein 6), also designated chemokine-like factor superfamily member 6, is a ubiquitously expressed transmembrane protein involved in immune regulation.
• It functions as a master regulator of PD-L1/CD274 recycling and plasma membrane expression, protecting PD-L1 from lysosomal degradation by preventing STUB1-mediated ubiquitination.
• CMTM6 associates with both constitutive and IFNG-induced PD-L1 at recycling endosomes, thereby enhancing PD-L1 stability and potentiating inhibitory signaling through the PDCD1/PD279 receptor on T-cells, ultimately triggering T-cell anergy.
• It is localized to the cell membrane, early endosome membrane, and recycling endosome membrane, consistent with its role in endosomal trafficking.
• Tissue expression is prominent in leukocytes, placenta, and testis, suggesting roles in immune privilege and reproductive immunology.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains MARVEL domains characteristic of membrane trafficking proteins.
• CMTM6 is a member of the chemokine-like factor superfamily, implicated in transport functions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide spanning amino acids 84-183 of human CMTM6, suggesting that the antibody may recognize an internal epitope. For Western blot (WB), a band around 20 kDa is expected; consider using positive controls such as human leukocyte lysates or cell lines with known CMTM6 expression.
• For ELISA applications, titrate the antibody and validate against recombinant CMTM6 or peptide antigens. Cross-reactivity with rat CMTM6 has been observed; further validation in rat samples can be performed if needed.
• Consider confirming specificity via siRNA knockdown or using isotype controls in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For projects targeting immune checkpoint molecules like CMTM6, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our network also facilitates sourcing rare-target raw materials, streamlining your supply chain for immuno-oncology assay development.