Anti-CMKLR1 Rabbit Polyclonal Antibody for WB and ELISA - Q99788

Core Product Specifications and Parameters

Biological Background: CMKLR1 Function and Localization

• CMKLR1, also known as Chemerin-like receptor 1, ChemR23, DEZ, ERV1, and RVER1, is a G protein-coupled receptor (GPCR) that mediates cellular responses to the chemoattractant adipokine chemerin/RARRES2 and the omega-3 fatty acid derivative resolvin E1.
• Upon ligand binding, CMKLR1 activates G(i)/G(o) proteins and beta-arrestin pathways, leading to intracellular calcium mobilization, MAP kinase (ERK1/2, p38) and PI3K phosphorylation; these signaling events regulate immune responses, adipogenesis, and angiogenesis.
  Related references:
  PMID:27716822
• In addition to its physiological role, CMKLR1 can act as a coreceptor for certain SIV strains (SIVMAC316, SIVMAC239, SIVMACL7E-FR, SIVSM62A) and primary HIV-1 (92UG024-2), highlighting its significance in viral entry.
• The receptor is localized at the cell membrane and is prominently expressed in developing bone and cartilaginous tissue, parathyroid glands, cardiovascular system, brain, kidney, gastrointestinal and myeloid tissues, as well as immune cells including monocyte-derived macrophages, immature dendritic cells, and endothelial cells; high expression is also found in differentiating adipocytes.
• CMKLR1 undergoes post-translational modifications such as glycosylation and phosphorylation, as indicated by keywords including glycoprotein, phosphoprotein, disulfide bond, and alternative splicing; its predicted molecular weight is approximately 42 kDa, though modifications may shift the observed band.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide mapping to residues 250-350 of human CMKLR1, an intracellular domain; sequence conservation in this region likely accounts for the reported cross‑reactivity with mouse. When working with other species, verify sequence alignment before use.
• In Western blotting, expect a major band at ~42 kDa; note that glycosylation and other modifications can result in multiple bands or a shifted migration. Use appropriate membrane preparations from cell lines or tissues expressing CMKLR1.
• For ELISA, optimization of coating conditions (e.g., antigen type, concentration) and antibody dilution is recommended. A peptide or recombinant protein corresponding to the immunogen may serve as a positive control.
• CMKLR1 is a membrane protein; considering this, enrich membrane fractions or use whole‑cell lysates from relevant biological samples (e.g., adipose tissue, leukocytes) for detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw‑material partner for diagnostic manufacturers, laboratories, and research institutes, offering a streamlined supply of validated antibodies, recombinant proteins, and ancillary reagents. This CMKLR1 polyclonal antibody is well‑suited for immunoassay development in metabolic, immunological, and infectious disease research. Beyond individual reagents, we support bulk procurement, custom antibody pairs, and rare‑target sourcing to help accelerate your project from concept to clinical application.