Anti-CMAS Rabbit Polyclonal Antibody for WB and ELISA - Q8NFW8

Core Product Specifications and Parameters

Biological Background: CMAS (N-acylneuraminate cytidylyltransferase) Function and Localization

• CMAS (CMP-N-acetylneuraminic acid synthase, also known as CSS) is a nucleotidyltransferase that catalyzes the activation of N-acetylneuraminic acid (NeuNAc) to CMP-NeuNAc, a key substrate for sialylation of glycoconjugates. It also exhibits low activity toward N-glycolylneuraminic acid (Neu5Gc) and 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN).
• The enzyme belongs to the transferase family, specifically the nucleotidyltransferases, and is essential for the biosynthesis of sialic acid‑containing glycans in the Golgi and nucleus.
• Alternative splicing of the CMAS gene generates multiple transcript variants, and the protein is subject to post‑translational modifications including acetylation and methylation, which may regulate its activity or localization.
• Subcellular localization is predominantly nuclear, consistent with its role in CMP‑sialic acid synthesis within the nucleus.
• CMAS is ubiquitously expressed across human tissues, with detection in pancreas, kidney, liver, skeletal muscle, lung, placenta, brain, heart, colon, peripheral blood leukocytes, small intestine, ovary, testis, prostate, thymus, and spleen.
• The calculated molecular weight of the full‑length protein is approximately 48.4 kDa (UniProt: 48379 Da), matching the observed band in Western blot applications.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein covering amino acids 1–263 of human CMAS. This N‑terminal region is highly conserved and is expected to generate antibodies recognizing the full‑length protein.
• For Western blotting, consider using a total protein load of 20–50 µg per lane and validate the band at ~48 kDa. Specificity can be confirmed by siRNA knockdown or CMAS‑knockout cell lysates if available.
• In ELISA, the recombinant immunogen or purified CMAS protein can serve as a positive control to verify antibody binding. Due to the polyclonal nature, lot‑to‑lot consistency should be monitored.
• The antibody cross‑reacts with mouse and rat CMAS; however, users should verify performance in their specific experimental systems due to potential species‑dependent epitope variations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and research institutions, offering streamlined access to a comprehensive portfolio of IVD raw materials, technical services, and consulting. This CMAS polyclonal antibody supports investigations into sialic acid metabolism, glycosylation disorders, and related pathways. Beyond this product, CamelBio can source validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and hard‑to‑find raw materials for your diagnostic development needs.