Anti-CLUH Rabbit Polyclonal Antibody (WB, IHC-P, IF/ICC, ELISA) - O75153

Core Product Specifications and Parameters

Biological Background: CLUH Function and Localization

• CLUH (clustered mitochondria protein homolog), also known as CLU1 and KIAA0664, is an mRNA-binding protein that plays a critical role in mitochondrial distribution and biogenesis.
• It specifically binds mRNAs of nuclear-encoded mitochondrial proteins in the cytoplasm, regulating their transport or translation in close proximity to mitochondria.
• The protein contains TPR repeats and features RNA-binding activity, as annotated by UniProtKB keywords.
• CLUH localizes to the cytoplasm and is enriched in cytoplasmic granules, consistent with its role in mRNA trafficking.
• Post-translational modifications include phosphorylation, as indicated by proteomics identification data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 1120–1309) of human CLUH, which may provide target specificity; however, validation in the desired sample system is recommended.
• For Western blot (WB), the predicted molecular weight is ~147 kDa; consider using an appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), ensure proper fixation and permeabilization to preserve antigenicity; include appropriate controls.
• Polyclonal antibodies may exhibit lot-to-lot variability; titrate each new lot for optimal performance in your assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers, laboratories, and research institutes with a one-stop supply of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects involving mitochondrial biology or RNA-binding proteins, our team can source rare-target raw materials and offer technical consulting to support your workflow from concept to clinic.