Anti-CLTA Polyclonal Antibody for WB, ELISA - P09496

Core Product Specifications and Parameters

Biological Background: Clathrin Light Chain A Function and Localization

• Clathrin light chain A (CLTA) is a key subunit of the clathrin triskelion, forming the polyhedral coat of coated pits and vesicles essential for receptor-mediated endocytosis and intracellular trafficking.
• As part of the TACC3/ch-TOG/clathrin complex, CLTA is implicated in stabilizing kinetochore fibers during mitosis by bridging adjacent microtubules. Related references: PMID:15858577 PMID:21297582
• Subcellularly, CLTA is found on cytoplasmic vesicle membranes, coated pits, the mitotic spindle, and the cytoskeleton, reflecting its dual role in membrane traffic and cell division.
• Post-translational modifications include acetylation and phosphorylation, with alternative splicing generating multiple isoforms that may fine-tune clathrin function.
• The protein contains calcium-binding motifs, hinting at calcium-regulated conformational changes or interactions (as indicated by the keyword "Calcium").

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against recombinant full-length human CLTA (aa 1-218); it is suitable for Western blot and ELISA as indicated.
• For WB, consider using whole-cell lysates from human, mouse, or rat cell lines known to express CLTA; a band near 27 kDa is expected.
• In ELISA, the antibody may be used as a capture or detection reagent; titrate and validate with the relevant antigen or sample system.
• Cross-reactivity with mouse and rat CLTA has been reported, but sequence homology suggests it may also recognize other species; validate independently.
• Note that clathrin cycling and post-translational modifications can influence antibody binding; ensure proper sample preparation (e.g., inclusion of phosphatase inhibitors).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide a seamless supply of IVD raw materials, from validated antibody pairs to rare target reagents. This anti-CLTA polyclonal antibody supports research into endocytosis, intracellular trafficking, and mitotic spindle regulation, complementing our portfolio of cytoskeleton and membrane traffic tools. Whether you need bulk ancillary reagents or custom sourcing, CamelBio bridges the gap from concept to clinic.