Anti-CLSPN (Claspin) Rabbit Polyclonal Antibody for WB, ELISA - Q9HAW4

Core Product Specifications and Parameters

Biological Background: Claspin (CLSPN) Function and Localization

• Claspin is a key mediator of the DNA damage response, required for cell cycle checkpoint arrest upon replication stress or genotoxic insults. Related references: PMID:12766152 PMID:15190204 PMID:15707391
• It acts as an adapter, binding BRCA1 and the checkpoint kinase CHEK1, and facilitates ATR-dependent phosphorylation of both proteins, thereby transducing damage signals. Related references: PMID:12766152 PMID:15096610 PMID:15707391
• Beyond checkpoint functions, Claspin maintains normal replication fork progression during unperturbed DNA synthesis and is essential for efficient replication.
• The protein directly binds DNA, showing preference for branched or forked structures, and interacts with replisome components including the MCM2-7 helicase complex and TIMELESS. Related references: PMID:15226314 PMID:34694004 PMID:35585232
• Claspin also plays a role in replication initiation by recruiting kinase CDC7 to phosphorylate MCM2-7 proteins. Related references: PMID:27401717
• The protein is localized to the nucleus and is subject to multiple post-translational modifications including acetylation, phosphorylation, and ubiquitination, which likely regulate its function.
• Alternative splicing generates multiple isoforms, and the protein contains predicted coiled-coil domains and repeat regions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 650-750 of human CLSPN (isoform NP_001177410.1); consider that epitope accessibility may vary across assays.
• For Western blotting, the full-length protein has a predicted molecular weight of ~151 kDa; validate band specificity using appropriate positive and negative controls, and note that alternative splicing or post-translational modifications may produce additional bands.
• ELISA performance should be optimized using recombinant protein or cell lysates; consider testing different coating conditions to confirm reactivity.
• When investigating DNA damage responses, ensure that the chosen cellular model exhibits relevant CLSPN expression and consider using synchronized cell populations to enhance detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CLSPN polyclonal antibody is well-suited for studies on DNA damage checkpoints, replication stress, and cell cycle regulation. Trust CamelBio for validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and expert support in sourcing rare targets for your IVD development needs.