Anti-CLPX Rabbit Monoclonal Antibody for WB, ELISA - O76031

Core Product Specifications and Parameters

Biological Background: CLPX Function and Localization

• ATP-dependent chaperone and unfoldase; part of the mitochondrial ClpXP protease complex, which recognizes, unfolds, and translocates specific protein substrates to the proteolytic subunit CLPP for degradation. Related references: PMID:11923310, PMID:22710082, PMID:28874591
• Functions as a chaperone in heme biosynthesis by facilitating the incorporation of pyridoxal phosphate cofactor into 5-aminolevulinate synthase, thereby activating ALA synthesis. Related references: PMID:28874591
• Involved in mitochondrial DNA (mtDNA) nucleoid distribution by regulating mitochondrial transcription factor A (TFAM) activity. Related references: PMID:22841477
• Also known as caseinolytic mitochondrial matrix peptidase chaperone subunit X; member of the ClpX chaperone family.
• Localizes to mitochondrion, mitochondrial matrix, and mitochondrion nucleoid.
• Post-translationally modified by acetylation and phosphorylation; contains ATP-binding and zinc-binding domains.
• Higher expression in skeletal muscle and heart; lower in liver, brain, placenta, lung, kidney, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 534–633). Consider that the antibody may detect full-length as well as processed forms.
• For Western blot, the expected band size is approximately 69 kDa. Validate with appropriate positive controls and consider the endogenous expression levels in your sample.
• For ELISA, the antibody can be explored as a capture or detection reagent. Titration and pairing studies are recommended.
• Cross-reactivity with mouse and rat has been demonstrated; verify performance in the relevant species and experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to a comprehensive portfolio of IVD raw materials. For mitochondrial targets like CLPX, we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in rare target raw-material sourcing—supporting your workflow from concept to clinic.