Anti-CLPTM1 Rabbit Polyclonal Antibody for WB and ELISA - O96005

Core Product Specifications and Parameters

Biological Background: CLPTM1 Function and Localization

• CLPTM1, also known as cleft lip and palate transmembrane protein 1, is a 76 kDa multi-pass membrane protein of the endoplasmic reticulum (ER).
• It functions as a putative lipid scramblase, potentially facilitating transbilayer lipid movement in the ER membrane (By similarity).
• CLPTM1 binds and retains GABAA receptors in the ER, thereby modulating inhibitory GABAergic neurotransmission by reducing receptor surface expression; altered signaling is implicated in cleft palate (By similarity).
• The protein is required for efficient glycosylphosphatidylinositol (GPI) inositol deacylation in the ER, a critical step for GPI-anchored protein maturation and trafficking. Related references: PMID:29255114
• CLPTM1 may also contribute to T-cell development (By similarity).
• It is widely expressed across tissues.
• Post-translational modifications include glycosylation, phosphorylation, and acetylation.
• Alternative splicing generates multiple CLPTM1 transcript variants.

Experimental Guidance and Technical Tips

• For Western blotting, CLPTM1 migrates at ~76 kDa. Use an 8-10% SDS-PAGE gel and standard wet transfer conditions; consider extended transfer times for high molecular weight proteins.
• The immunogen spans the N-terminal 280 amino acids, covering most of the protein's luminal/extracellular domains; this design may enhance detection of both native and denatured forms in WB and ELISA.
• In ELISA, the recombinant immunogen fragment can be used as a coating antigen for assay development and antibody titration.
• As CLPTM1 is an integral membrane protein, include mild detergents (e.g., 0.1% Triton X-100) in lysis buffers to ensure efficient solubilization.
• The antibody shows cross-reactivity with mouse and rat orthologs; validate in the relevant species and tissue type, as expression may vary.
• Being a polyclonal antibody, it offers robust signal amplification; however, ensure reagent consistency by establishing acceptance criteria for new lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and R&D labs from target discovery to clinical application. For research on GABAergic signaling, lipid scrambling, or GPI anchor biology, we supply this anti-CLPTM1 polyclonal antibody along with a broad portfolio of validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents. Our team can also assist in sourcing rare-target raw materials and optimizing reagent supply chains to accelerate your assay development and production.