Anti-CLPTM1 Rabbit Monoclonal Antibody for WB and ELISA - O96005

Core Product Specifications and Parameters

Biological Background: CLPTM1 Function and Localization

• CLPTM1, also known as cleft lip and palate transmembrane protein 1, is a putative lipid scramblase that is widely expressed in human tissues.
• It plays a role in GABAergic transmission by binding and trapping GABAA receptors in the endoplasmic reticulum (ER), thereby reducing their plasma membrane expression and modulating inhibitory neurotransmission; altered GABAergic signaling can contribute to cleft palate (By similarity).
• CLPTM1 may function as a lipid scramblase, translocating lipids between membrane leaflets (By similarity).
• The protein is required for efficient glycosylphosphatidylinositol (GPI) inositol deacylation in the ER, a crucial step for the maturation of GPI-anchored proteins. Related references: PMID:29255114
• It localizes to the ER membrane, consistent with its roles in protein retention and GPI processing.
• Post-translational modifications include acetylation and phosphorylation, and the protein exists in alternatively spliced isoforms; it is a glycoprotein with a molecular weight of approximately 76 kDa.
• CLPTM1 may also play a role in T-cell development (By similarity).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (aa 1-100) of human CLPTM1; therefore, the antibody is expected to recognize the full-length protein and possibly N-terminal fragments or isoforms containing this region. For WB, a band near 76 kDa is anticipated.
• For ELISA, consider using the immunogen peptide as a positive control to validate antibody binding.
• Given the exclusive cross-reactivity with human, this antibody is suitable for studies in human cell lines and tissues. For species other than human, validation is recommended.
• For WB applications, optimize lysate preparation and antibody dilution in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supplying high-quality antibodies like this anti-CLPTM1 mAb for research on neurological disorders and protein trafficking. We offer validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and support for rare target sourcing to accelerate your assay development from concept to clinic.