Anti-CLPS (Colipase) Rabbit Polyclonal Antibody for WB and ELISA - P04118

Core Product Specifications and Parameters

Biological Background: Colipase Function and Localization

• Colipase, encoded by the CLPS gene, is a small secreted protein cofactor essential for pancreatic lipase activity in dietary fat digestion.
• It enables pancreatic lipase to anchor at the lipid-water interface, preventing its displacement by bile salts.
• The protein is primarily expressed in the pancreas and secreted into the intestinal lumen.
• Enterostatin, a pentapeptide released from the N-terminal region of colipase during digestion, acts as a satiety signal, contributing to appetite regulation.
• Colipase contains disulfide bonds critical for its structural stability and functional conformation.
• The molecular weight of mature colipase is approximately 12 kDa (precisely 11.95 kDa based on sequence).

Experimental Guidance and Technical Tips

• The immunogen was designed as a synthetic peptide spanning amino acids 13–112 of human CLPS (NP_001823.1), covering most of the mature protein.
• For Western blot, the predicted band size is around 12 kDa; ensure appropriate gel concentration and transfer conditions for small proteins.
• The antibody has shown cross-reactivity with mouse and rat colipase, likely due to sequence conservation; validate in your species and tissue of interest.
• For ELISA, consider pairing with a matched detection antibody or using as a capture/detection reagent depending on assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, providing access to validated antibodies, recombinant proteins, and ancillary reagents for diagnostic and research applications. Whether you need bulk anti-colipase antibodies for digestive health assay development, or custom antibody generation against lipid metabolism targets, we can support your program from concept to clinic.