Anti-CLPP Rabbit Polyclonal Antibody for WB, ELISA - Q16740

Core Product Specifications and Parameters

Biological Background: CLPP Function and Localization

• The CLPP gene encodes the ATP-dependent Clp protease proteolytic subunit, mitochondrial, also known as caseinolytic mitochondrial matrix peptidase proteolytic subunit or endopeptidase Clp.
• CLPP is the proteolytic component of the ClpXP complex, which degrades a broad range of substrates including caseins (CSN1S1, CSN2, CSN3) and PINK1, suggesting a central housekeeping role in mitochondrial protein quality control. Related references: PMID:11923310 PMID:15522782 PMID:22354088
• It localizes to the mitochondrial matrix.
• Tissue expression is highest in skeletal muscle, with intermediate levels in heart, liver, and pancreas, and low in brain, placenta, lung, and kidney; protein detected in liver.
• Post-translational modifications include acetylation; it belongs to the serine protease family and is associated with deafness (DFNB81) and other disease variants.

Experimental Guidance and Technical Tips

• For Western blotting, consider using mitochondrial-enriched lysates to enhance detection of the 30 kDa CLPP protein. Validate antibody specificity with appropriate positive and negative controls.
• For ELISA, test against the recombinant immunogen (amino acids 57-277 of human CLPP) to confirm reactivity and assess cross-reactivity with other species.
• As a polyclonal antibody, lot-to-lot consistency should be verified in your specific assay system, and optimal working dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers researchers and diagnostic manufacturers a one-stop sourcing platform for IVD raw materials, from concept to clinic. This anti-CLPP rabbit polyclonal antibody supports mitochondrial research and assay development, and can be complemented by validated antibody pairs, recombinant proteins, and ancillary reagents. For specialized targets like CLPP and rare mitochondrial markers, CamelBio’s supply chain expertise ensures reliable procurement and technical support.