Anti-CLPB Rabbit Polyclonal Antibody for IHC-P, ELISA - Q9H078

Core Product Specifications and Parameters

Biological Background: CLPB Function and Localization

• CLPB (caseinolytic peptidase B protein homolog) is a mitochondrial disaggregase that functions as a regulatory ATPase, essential for secretion and intracellular protein trafficking.
• It exhibits ATP-dependent protein disaggregase activity, which is required to maintain the solubility of key mitochondrial proteins. Related references: PMID:32573439, PMID:34115842, PMID:35247700
• CLPB is involved in mitochondrial-mediated antiviral innate immunity; it activates RIG-I-mediated signal transduction, leading to the production of type I interferon (IFNB1) and pro-inflammatory cytokine IL6. Related references: PMID:31522117
• The protein plays a role in granulocyte differentiation. Related references: PMID:34115842
• Subcellularly, CLPB localizes to the mitochondrial intermembrane space.
• It is widely expressed at the protein level, with highest expression in adult brain regions such as the thalamus, hippocampus, and cortex, and lower levels in granulocytes.
• Structurally, CLPB contains ANK repeats and a nucleotide-binding domain; mutations in the gene are associated with various diseases, including cataract, epilepsy, and intellectual disability.

Experimental Guidance and Technical Tips

• As a polyclonal antibody generated against a recombinant fragment of human CLPB, it may detect multiple isoforms due to alternative splicing. Verify specificity in your sample type.
• For IHC-P, consider antigen retrieval and appropriate tissue fixation; validate on positive control tissues such as brain or mitochondrial-rich samples.
• For ELISA, titrate the antibody to determine the optimal working concentration for your specific assay format.
• Cross-reactivity with mouse and rat CLPB enables use in commonly studied rodent models, but sequence homology should be confirmed for your target region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and research institutions with validated antibody pairs, bulk ancillary reagents, and customized procurement for challenging targets like CLPB. With expertise in mitochondrial biology and antiviral research, we help secure consistent supplies of high-quality reagents to accelerate your assay development from concept to clinic.