Anti-CLNS1A Rabbit Polyclonal Antibody for WB, ELISA - P54105

Core Product Specifications and Parameters

Biological Background: CLNS1A Function and Localization

• CLNS1A encodes the methylosome subunit pICln, also known as chloride conductance regulatory protein ICln, reticulocyte pICln, and chloride ion current inducer protein ClCI.
• It is a chaperone critical for the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome essential for pre-mRNA splicing. Related references: PMID:10330151 | PMID:18984161
• pICln traps Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF, and SNRPG in an inactive 6S pICln-Sm complex in the cytosol, controlling the assembly of the core snRNP. Related references: PMID:10330151 | PMID:18984161
• The SMN complex dissociates pICln from the trapped Sm proteins and transfers them to an SMN-Sm complex, triggering core snRNP assembly and nuclear transport. Related references: PMID:10330151 | PMID:18984161
• Additionally, CLNS1A is involved in the methylation of Sm proteins, a modification important for snRNP biogenesis. Related references: PMID:11713266
• Subcellularly, pICln localizes to the cytoplasm, cytosol, nucleus, and associates with the cytoskeleton, reflecting its dynamic role in snRNP trafficking.
• The protein undergoes post-translational modifications, including acetylation and phosphorylation, and has a molecular weight of approximately 26 kDa (26215 Da per UniProt).
• UniProt Keywords: 3D-structure, Acetylation, Cytoplasm, Cytoskeleton, Direct protein sequencing, mRNA processing, mRNA splicing, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against full-length recombinant human CLNS1A (amino acids 1–237). For Western blot, consider using a standard blocking buffer and HRP-conjugated anti-rabbit secondary antibody; the predicted band is ~26 kDa.
• For ELISA, the antibody can be used as a capture or detection reagent; titration and optimization in the relevant assay system are recommended.
• Cross-reactivity is reported for human; perform validation in your specific human cell lines or tissue lysates.
• As a polyclonal antibody, it may recognize multiple epitopes, which can enhance signal but also requires careful specificity controls (e.g., knockout cell lines or peptide competition).
• The antibody is suitable for studying spliceosome dynamics, snRNP assembly, and Sm protein methylation pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering diagnostic manufacturers and research laboratories streamlined access to high-quality antibodies, antigens, and ancillary reagents. This anti-CLNS1A polyclonal antibody is part of our expanding portfolio targeting mRNA splicing and snRNP biology. Leverage our supply capabilities for validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare-target raw materials to support your diagnostic and research workflows from concept to clinic.