Anti-CLNS1A Polyclonal Antibody for WB, IHC-P, ELISA - P54105

Core Product Specifications and Parameters

Biological Background: CLNS1A Function and Localization

• CLNS1A encodes methylosome subunit pICln, also known as chloride conductance regulatory protein ICln, which acts as a chaperone for spliceosomal snRNP assembly. Related references: PMID:10330151, PMID:18984161
• It regulates the assembly of U1, U2, U4 and U5 snRNPs by trapping Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG in an inactive 6S pICln-Sm complex in the cytosol. Related references: PMID:10330151
• Upon dissociation by the SMN complex, Sm proteins are transferred to the SMN-Sm complex, triggering core snRNP assembly and nuclear transport. Related references: PMID:10330151, PMID:18984161
• CLNS1A is also involved in the methylation of Sm proteins, contributing to snRNP biogenesis. Related references: PMID:11713266
• It localizes to the cytoplasm, cytosol, nucleus, and cytoskeleton, reflecting its dynamic role in snRNP trafficking.
• Post-translational modifications include acetylation and phosphorylation, which may regulate its function.
• It is identified by direct protein sequencing and mass spectrometry-based proteomics, confirming endogenous expression.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight is 26kDa; consider using a positive control lysate from human, mouse, or rat cells to confirm specificity.
• For IHC-P, optimize antigen retrieval conditions and validate the antibody in tissue sections known to express CLNS1A.
• The immunogen covers the full-length protein (aa 1-237), so the antibody likely recognizes native and denatured forms; consider epitope mapping if precise binding information is required.
• Since CLNS1A functions in snRNP assembly, co-immunoprecipitation may be useful for interaction studies; validate the antibody for IP if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing bridge for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For RNA splicing and snRNP assembly studies, we offer validated anti-CLNS1A polyclonal antibodies as well as complementary reagents like recombinant proteins and antibody pairs. Our flexible supply chain ensures consistent bulk availability of high-quality reagents tailored to your assay development needs.