Anti-CLN5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75503

Core Product Specifications and Parameters

Biological Background: CLN5 Function and Localization

• Also known as ceroid-lipofuscinosis neuronal protein 5, CLN5 is a lysosomal membrane protein involved in lipid metabolism and protein depalmitoylation.
• Catalyzes the synthesis of bis(monoacylglycero)phosphate (BMP) via transacylation of lysophosphatidylglycerol, playing a key role in intraluminal vesicle formation and cholesterol homeostasis. Related references: PMID:37708259
• Exhibits BMP synthase activity towards various lysophosphatidylglycerol species with preference for longer chain lengths. Related references: PMID:37708259
• Influences retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from endosomes to the trans-Golgi network by controlling retromer recruitment. Related references: PMID:22431521
• Regulates the localization and activation of RAB7A, a GTPase required for retromer complex recruitment to endosomal membranes. Related references: PMID:22431521
• Possesses palmitoyl protein thioesterase (S-depalmitoylase) activity in vitro, likely contributing to protein S-depalmitoylation.
• Subcellular localization is primarily at the lysosome membrane.
• Ubiquitously expressed across tissues.
• Mutations in CLN5 are associated with neuronal ceroid lipofuscinosis (CLN5 disease), a neurodegenerative lysosomal storage disorder characterized by progressive neuronal loss.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of CLN5 is approximately 41 kDa; ensure proper gel percentage and transfer conditions for optimal detection.
• In immunohistochemistry (IHC-P), antigen retrieval methods and antibody dilution may require optimization for different tissue types; include appropriate positive and negative controls.
• As a polyclonal antibody, lot-to-lot consistency should be validated, and we recommend titrating the antibody for your specific assay.
• For ELISA, this antibody can be utilized as a capture or detection reagent; perform checkerboard titrations to establish optimal coating and detection concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, providing this anti-CLN5 antibody alongside validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing to support diagnostic and research applications in neurodegeneration and lysosomal storage disorders. Our portfolio can be complemented with matched detection reagents, enabling streamlined development of assays for neuronal ceroid lipofuscinosis research.