Anti-CLIP1 Rabbit Polyclonal Antibody for WB, ELISA - P30622

Core Product Specifications and Parameters

Biological Background: CLIP1 Function and Localization

• CAP-Gly domain-containing linker protein 1 (CLIP1), also known as CLIP-170, restin, or cytoplasmic linker protein 1, is a microtubule plus-end tracking protein.
• It binds to the plus end of microtubules and regulates microtubule dynamics by promoting growth and bundling.
• CLIP1 links cytoplasmic vesicles to microtubules, playing a critical role in intracellular vesicle trafficking, macropinocytosis, and endosome trafficking.
• The protein contains CAP-Gly domains, coiled-coil regions, and zinc-binding motifs, contributing to its structural and functional versatility.
• Subcellularly, CLIP1 localizes to the cytoplasm, microtubule cytoskeleton, cytoplasmic vesicle membranes, and cell projection ruffles.
• It is detected in dendritic cells and highly expressed in the Reed-Sternberg cells of Hodgkin disease.
• CLIP1 is a phosphoprotein, and post-translational modifications likely regulate its function.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1228–1427 of human CLIP1; consider sequence alignment to assess conservation across species of interest.
• For Western blot, use a low‑percentage polyacrylamide gel (e.g., 6–8%) to adequately resolve the high molecular weight protein (~162 kDa); extend transfer times accordingly.
• Validate antibody specificity in your target sample system (e.g., lysates from human, mouse, or rat cells) to confirm cross‑reactivity.
• Depending on your assay, optimize antibody dilutions for Western blot or ELISA using standard protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers a streamlined, one-stop solution for IVD raw materials. For microtubule and vesicle trafficking research, our portfolio includes validated antibodies like this anti‑CLIP1 polyclonal antibody, along with bulk ancillary reagents and customized sourcing support. Let CamelBio bridge the gap from target discovery to diagnostic application with reliable, cost‑effective materials and technical expertise.