Anti-CLIP1 Polyclonal Antibody for WB, ELISA - P30622

Core Product Specifications and Parameters

Biological Background: CLIP1 Function and Localization

• CAP-Gly domain-containing linker protein 1 (CLIP1), also known as CLIP-170 or restin, is a microtubule plus-end tracking protein (+TIP) that regulates cytoskeleton dynamics.
• It promotes microtubule growth and bundling, playing a critical role in maintaining microtubule structure and function.
• CLIP1 links cytoplasmic vesicles to microtubules, facilitating intracellular vesicle trafficking, macropinocytosis, and endosome transport.
• The protein localizes to the cytoplasm, cytoskeleton, cytoplasmic vesicle membranes, and cell projections such as ruffles.
• It is highly expressed in Reed-Sternberg cells of Hodgkin disease and detected in dendritic cells at the protein level, indicating potential roles in immune cell function and cancer.
• CLIP1 contains coiled-coil regions, zinc-finger motifs, and metal-binding domains, and is subject to phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal fragment (amino acids 1228–1427) present in major CLIP1 isoforms. Consider epitope accessibility when using this antibody in immunofluorescence or immunohistochemistry.
• For Western blot, expect a band around 162 kDa. Use a low‑percentage gel (e.g., 6–8%) and extended transfer times to ensure efficient detection of high‑molecular‑weight proteins.
• As a polyclonal antibody, it provides robust signal in ELISA; however, careful blocking and optimization of coating conditions may be required to minimize background.
• Cross‑reactivity with mouse and rat has been demonstrated, making it suitable for rodent model studies. Sequence alignment in the immunogen region is recommended before use in other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibodies, bulk ancillary reagents, and specialized sourcing services. This anti‑CLIP1 polyclonal antibody supports investigations into cytoskeleton dynamics, vesicle trafficking, and oncology—particularly Hodgkin disease and dendritic cell biology. Whether you need optimized antibody pairs, large‑scale production, or rare target reagent access, CamelBio bridges your project from concept to clinic with reliable, one‑stop support.