Anti-CLIC4 Rabbit Monoclonal Antibody for WB and ELISA - Q9Y696

Core Product Specifications and Parameters

Biological Background: CLIC4 Function and Localization

• CLIC4, also known as p64H1 or mtCLIC, is a member of the chloride intracellular channel (CLIC) family, existing in both soluble and integral membrane forms.
• In its soluble state, CLIC4 functions as a glutaredoxin-like oxidoreductase, catalyzing thiol-disulfide exchange reactions using reduced glutathione as an electron donor. Related references: PMID:25581026 PMID:37759794
• Under oxidizing conditions, CLIC4 can insert into membranes to form voltage-dependent, multi-ion conductive channels; membrane insertion is redox-regulated. Related references: PMID:16176272
• CLIC4 plays roles in angiogenesis, endothelial cell proliferation, and maintenance of apical-basolateral polarity during mitosis and cytokinesis.
• The protein localizes to multiple subcellular compartments, including the cytoplasm, cytoskeleton, centrosomes, cytoplasmic vesicles, nucleus, cell membrane, mitochondria, cell junctions, and endoplasmic reticulum.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics.
• CLIC4 is prominently expressed in heart, kidney, placenta, and skeletal muscle, and is detected in epithelial cells from colon, esophagus, and kidney.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–100) of human CLIC4. This region may be effective for detecting both soluble and membrane-inserted forms; validate specificity in your experimental system.
• For Western blot, expect a band at approximately 29 kDa under reducing conditions. Use appropriate positive controls (e.g., HeLa or HEK293 lysates) and confirm with known expression patterns.
• In ELISA, this antibody can be used for direct antigen detection or as a capture/detection reagent. Consider compatibility with your assay format and sample matrix.
• Reported cross-reactivity with mouse and rat CLIC4; verify sequence homology for your application.
• Since CLIC4 undergoes redox-dependent conformational changes, consider optimizing reducing/oxidizing conditions in your assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and life science researchers with one-stop access to IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For ion channel and redox biology projects, we offer optimized monoclonal antibodies and technical consulting to accelerate your assay development. Contact us to source custom CLIC4 reagents or ancillary materials for your workflow.