Anti-CLIC1 Rabbit Polyclonal Antibody for WB, ELISA - O00299

Core Product Specifications and Parameters

Biological Background: CLIC1 Function and Localization

• Chloride intracellular channel protein 1 (CLIC1), also known as NCC27, G6, or CL1C1, belongs to the CLIC family of multifunctional proteins. In its soluble form, it functions as a glutaredoxin-like oxidoreductase.
  Related references:
  PMID:25581026
  PMID:37759794
• Under oxidizing conditions, CLIC1 can insert into membranes to form voltage-dependent chloride ion channels, playing a role in ion homeostasis and cell volume regulation.
• The protein shuttles between the cytoplasm, nucleus, endoplasmic reticulum, and cell membrane, with prominent nuclear membrane localization under certain conditions.
• CLIC1 is broadly expressed, with highest levels in heart, placenta, liver, kidney, and pancreas.
• Post-translational modifications include acetylation, phosphorylation, and glutathionylation, which may regulate its activity and subcellular localization.
• CLIC1 is implicated in cell cycle regulation, potentially through its ion channel activity or redox functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 42–241, representing the majority of the protein, and is expected to detect both soluble and membrane-inserted forms.
• For Western blot, the predicted molecular weight is 27 kDa; however, post-translational modifications or membrane insertion may slightly shift the electrophoretic mobility.
• Validate antibody specificity in your target tissue using appropriate positive (e.g., heart, placenta) and negative controls.
• For ELISA, consider pairing with a matched detection antibody to build a sandwich assay; epitope accessibility may vary with sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibodies, including this CLIC1 polyclonal antibody, alongside bulk reagents and rare-target sourcing for ion channel and redox biology studies. Our integrated supply chain helps diagnostic developers and researchers streamline procurement from concept to clinic.