Anti-CLEC7A Polyclonal Antibody for WB, ELISA - Q9BXN2

Core Product Specifications and Parameters

Biological Background: CLEC7A Function and Localization

• CLEC7A (Dectin-1) is a member of the C-type lectin family, also known as beta-glucan receptor, CLECSF12, and DC-associated C-type lectin 1.
• It functions as a pattern recognition receptor (PRR) that specifically recognizes beta-1,3- and beta-1,6-linked glucans from fungal and bacterial cell walls. Related references: PMID:11567029 PMID:12423684
• Upon ligand binding, CLEC7A recruits SYK via its ITAM motif, leading to activation of NF-kappa-B and MAP kinase pathways, which induce pro-inflammatory cytokines and chemokines.
• It mediates phagocytosis of Candida albicans conidia and enhances cytokine production in macrophages and dendritic cells. Related references: PMID:17230442
• CLEC7A is also involved in T-cell activation, binding to T-cells independently of surface glycans and stimulating their proliferation.
• Subcellularly, the protein localizes to the cell membrane and cytoplasm. It is highly expressed in peripheral blood leukocytes, dendritic cells, spleen, bone marrow, lung, muscle, stomach, and placenta.
• Key structural features include disulfide bonds, glycosylation, and a transmembrane domain, reflecting its role as a signaling receptor in innate immunity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 140–240 of human CLEC7A (NP_922938.1); verify epitope accessibility under your experimental conditions.
• For Western blot, anticipate a band near 28 kDa (predicted MW ~28 kDa; UniProt: 27.6 kDa). Run appropriate positive controls (e.g., leukocyte or dendritic cell lysates).
• This antibody can be used in ELISA; consider optimizing coating antigen and antibody dilution through checkerboard titrations.
• Note that cross-reactivity with mouse and rat has been reported; confirm specificity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, including this polyclonal antibody targeting CLEC7A, an important innate immunity receptor. Beyond individual reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets, supporting diagnostic manufacturers and research laboratories from concept to clinic.