Anti-CLEC4E Polyclonal Antibody for WB, ELISA - Q9R0Q8

Core Product Specifications and Parameters

Biological Background: CLEC4E (Mincle) Function and Localization

• CLEC4E, also known as C-type lectin domain family 4 member E, macrophage-inducible C-type lectin (Mincle), or C-type lectin superfamily member 9, is a calcium-dependent lectin and pattern recognition receptor (PRR) of the innate immune system. Related references: PMID:18490740, PMID:18509109
• It recognizes damage-associated molecular patterns (DAMPs) from abnormal self and pathogen-associated molecular patterns (PAMPs) from bacteria and fungi, including mycobacterial trehalose 6,6'-dimycolate (TDM) and fungal alpha-mannose residues. Related references: PMID:18776906, PMID:20008526, PMID:23602766, PMID:19171887
• Upon ligand binding, CLEC4E associates with the Fc receptor gamma chain (FCER1G) to form a signaling complex, leading to ITAM phosphorylation and activation of SYK, CARD9, and NF-kappa-B pathways, thereby promoting maturation of antigen-presenting cells and Th1/Th17 polarization. Related references: PMID:23602766
• CLEC4E localizes to the cell membrane, cell projections, and the phagocytic cup, consistent with its role in immune surveillance and phagocytosis.
• It is highly expressed in macrophages and dendritic cells upon stimulation with bacterial glycolipids or pro-inflammatory cytokines, linking innate sensing to adaptive immunity. Related references: PMID:10528209, PMID:23602766
• The protein contains structural features typical of C-type lectins, including a transmembrane helix, a signal-anchor, disulfide bonds, and glycosylation sites, and it binds calcium and other metals essential for carbohydrate recognition.
• Gene family: CLEC4E belongs to the C-type lectin superfamily. Its mouse gene ID is 56619, and the human ortholog is 26253.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 120–200 of mouse CLEC4E (NP_064332.1), corresponding to the predicted extracellular C-type lectin domain. This region is expected to be exposed on the cell surface and accessible for antibody binding.
• For Western blot (WB), the predicted molecular weight is ~24 kDa; consider running appropriate positive controls such as stimulated macrophage lysates.
• In ELISA, the antibody may capture and detect recombinant or native CLEC4E; validate assay conditions using established antigen preparations.
• The antibody shows cross-reactivity with rat, so it may be suitable for comparative studies. Optimize dilutions for each species and application.
• As a polyclonal antibody, it may recognize multiple epitopes, providing robust signal but potential batch-to-batch variation; consider lot-specific validation in critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-CLEC4E polyclonal antibody supports innate immunity and pattern recognition research, backed by CamelBio’s commitment to reliable supply and technical compatibility. Leverage our integrated sourcing capabilities to accelerate your assay development from concept to clinic.