Anti-CLEC4D Polyclonal Antibody for WB, IF-P, ELISA - Q8WXI8

Core Product Specifications and Parameters

Biological Background: CLEC4D Function and Localization

• Also known as Dectin-3, CD368, and CLECSF8, CLEC4D is a member of the C-type lectin family that functions as a pattern recognition receptor (PRR) in the innate immune system.
• CLEC4D recognizes damage-associated molecular patterns (DAMPs) and pathogen-associated molecular patterns (PAMPs) from bacteria and fungi, including α-mannans on Candida albicans hyphae and mycobacterial trehalose 6,6'-dimycolate (TDM). Related references: PMID:23602766 PMID:23911656
• Upon ligand engagement, CLEC4D likely forms a complex with Fc receptor gamma chain (FCER1G) via CLEC4E (Mincle), leading to phosphorylation of the ITAM motif, SYK-CARD9-NF-kB activation, and subsequent maturation of antigen-presenting cells with induction of T-helper 1 and 17 responses. Related references: PMID:23602766 PMID:23911656
• CLEC4D acts as an endocytic receptor that may facilitate antigen uptake at sites of infection for clearance or for processing and presentation to T cells. Related references: PMID:14971047
• Subcellularly, CLEC4D localizes to the cell membrane as a transmembrane protein with a single signal-anchor helix.
• Tissue expression is predominantly in monocytes and macrophages, and can be upregulated by cytokines such as IL-6, IL-10, TNF, and IFN-gamma.
• Key post-translational modifications include N-linked glycosylation and disulfide bond formation, contributing to its structural stability and ligand-binding properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 39–215 of human CLEC4D (NP_525126.2); thus, the polyclonal antibody may recognize the extracellular ligand-binding domain and potentially the stalk region. Consider that glycosylation might influence epitope accessibility.
• For Western blotting, the predicted molecular weight is ~25 kDa, but glycosylated forms may migrate higher. Use appropriate positive controls such as THP-1 cells stimulated with cytokines (e.g., TNF or IFN-gamma) to enhance CLEC4D expression.
• In immunofluorescence (IF-P), the antibody is expected to detect membrane-localized CLEC4D. Permeabilization is required if the epitope is intracellular; otherwise, surface staining on live, unfixed cells may be evaluated.
• For ELISA, ensure that the recombinant protein or native antigen is properly coated and consider cross-reactivity with mouse and rat homologs when designing assays.
• As with any polyclonal antibody, lot-to-lot consistency should be monitored, and specificity can be verified using CLEC4D knockout or knockdown samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies a comprehensive portfolio of IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents for immunodiagnostic development. This anti-CLEC4D antibody supports research into innate immunity and infectious disease diagnostics. Leveraging our one-stop platform, diagnostic manufacturers gain streamlined access to bulk antibody pairs, optimized monoclonal/polyclonal antibodies, and rare-target sourcing services—empowering your transition from concept to clinic.