Anti-CLEC12A Polyclonal Antibody for WB, IF-P, ELISA - Q5QGZ9

Core Product Specifications and Parameters

Biological Background: CLEC12A Function and Localization

• CLEC12A, also known as CLL-1, MICL, CD371, DCAL-2, is a C-type lectin domain family member encoded by the CLEC12A gene (Gene ID: 160364) in humans. It functions as a myeloid inhibitory receptor that negatively regulates myeloid cell activation and limits proinflammatory pathways. Related references: PMID:14739280, PMID:15238421, PMID:16239426
• The protein acts as a pattern-recognition receptor (PRR) for pathogen-associated molecules, including plasmodium hemozoin and mycobacterial mycolic acid, mediating immune responses against infections. Related references: PMID:31269448, PMID:36542980
• CLEC12A also recognizes endogenous ligands such as neutrophil extracellular traps (NETs) and monosodium urate crystals, inhibiting neutrophil activation and limiting NET formation, playing a role in controlling inflammatory conditions. Related references: PMID:38367667, PMID:38386511, PMID:39143217
• Ligand binding induces phosphorylation of its immunoreceptor tyrosine-based inhibition motif (ITIM), leading to recruitment of phosphatases PTPN6 and PTPN11, which counteract SYK signaling, thereby preventing cell activation. Related references: PMID:14739280, PMID:16239426, PMID:34234773
• The receptor is expressed on the cell membrane of various immune cells, including natural killer (NK) cells, T-cells, dendritic cells, monocytes, and macrophages, with preferential expression in lymphoid tissues such as spleen and lymph node. Related references: PMID:14739280, PMID:15238421, PMID:15548716, PMID:16239426, PMID:16838277
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which are essential for its structure and function. It also undergoes alternative splicing, producing multiple isoforms.
• CLEC12A inhibits NK cell cytotoxicity and dendritic cell maturation in an IL10-dependent manner, highlighting its broad immunoregulatory roles. Related references: PMID:15238421, PMID:16239426
• The predicted molecular weight of human CLEC12A is approximately 31 kDa (30762 Da).

Experimental Guidance and Technical Tips

• The immunogen (amino acids 66-265) covers the extracellular C-type lectin-like domain of human CLEC12A; this region is conserved across species, supporting the reported cross-reactivity with mouse and rat.
• For Western blot (WB), use whole-cell lysates from immune cell lines (e.g., THP-1, U937) or primary myeloid cells; CLEC12A may appear as a ~31 kDa band, but glycosylation could cause slight molecular weight shifts.
• In immunofluorescence (IF-P), ensure permeabilization and appropriate fixation to detect membrane-bound CLEC12A; co-staining with myeloid markers may confirm specificity.
• ELISA applications may benefit from pairing with a matched detection antibody; validate the antibody in the relevant sample matrix and buffer conditions.
• Always titrate the antibody to determine the optimal working dilution for your specific experimental setup, as the provided applications are supplier-reported.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for IVD raw material sourcing, connecting diagnostic manufacturers and research laboratories with validated antibodies, recombinant proteins, and ancillary reagents. This anti-CLEC12A polyclonal antibody expands our portfolio for immune regulation and myeloid cell research, supporting immunoassay development, biomarker discovery, and infection/inflammation studies. From rare-target sourcing to bulk supply, CamelBio streamlines your path from concept to clinic, enabling you to accelerate diagnostic innovation with confidence.