Anti-Cleaved PARP (Asp214) Monoclonal Antibody for WB, IF/ICC, ELISA - P09874

Core Product Specifications and Parameters

Biological Background: PARP1 Function and Localization

• PARP1 is a nuclear poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of substrate proteins, playing a central role in DNA repair, chromatin remodeling, and genome stability (PubMed:17177976, PubMed:18055453, PubMed:18172500). Related references: PMID:17177976 PMID:18055453 PMID:18172500
• It catalyzes serine-, glutamate-, and tyrosine-linked ADP-ribosylation, with residue specificity determined by cofactors HPF1 and NMNAT1 (PubMed:28190768, PubMed:32028527, PubMed:29954836). Related references: PMID:28190768 PMID:32028527 PMID:29954836
• In DNA repair, PARP1 senses strand breaks, recruits repair factors, and promotes chromatin relaxation to facilitate base excision and double-strand break repair (PubMed:26344098, PubMed:30356214, PubMed:19344625). Related references: PMID:26344098 PMID:30356214 PMID:19344625
• During apoptosis, PARP1 is cleaved by caspase-3 and -7 at Asp214, generating a large catalytic fragment (∼89 kDa) that translocates to the cytoplasm and serves as a poly-ADP-ribose carrier, triggering AIFM1-mediated apoptosis (PubMed:33168626). Related references: PMID:33168626
• Subcellular localization is predominantly nuclear and nucleolar under normal conditions; upon cleavage, the catalytic fragment relocates to the cytoplasm.
• PARP1 undergoes extensive post-translational modifications, including acetylation, ADP-ribosylation (automodification), phosphorylation, and ubiquitination, which regulate its activity and interactions.
• Beyond DNA repair, PARP1 functions in transcription regulation, replication fork progression, adipogenesis, and innate immunity by modulating protein activities and chromatin structure (PubMed:15607977, PubMed:22464733, PubMed:35460603). Related references: PMID:15607977 PMID:22464733 PMID:35460603

Experimental Guidance and Technical Tips

• This antibody targets the neo-epitope at Asp214; thus, it specifically detects the cleaved PARP fragment rather than full-length PARP. For western blot, treat cells with apoptosis inducers (e.g., staurosporine or etoposide) to enrich the ∼89 kDa fragment.
• For immunofluorescence/ICC, consider fixation after apoptosis induction to observe cytosolic localization of the cleaved fragment. Validate staining specificity in your experimental system with appropriate controls.
• ELISA suitability has been reported; when designing assays, consider using recombinant cleaved PARP fragment or peptide as a standard and optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated access to high-quality IVD raw materials, supporting your DNA damage and apoptosis research or diagnostic development. We specialize in supplying validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and rare-target sourcing, streamlining your path from concept to clinic.