Anti-Cleaved Gasdermin D (N-Terminal) Rabbit Monoclonal Antibody for Pyroptosis Research - P57764

Core Product Specifications and Parameters

Biological Background: Gasdermin D Function and Localization

• Precursor of a pore-forming protein that, upon cleavage by inflammatory caspases, releases an N-terminal fragment that binds membranes and forms pores, triggering pyroptosis. Related references: PMID:26375003 PMID:26375259 PMID:27281216
• Promotes pyroptosis in response to microbial infection and danger signals; cleaved by CASP1, CASP4, or CASP5; translocates to the plasma membrane, binds inner leaflet lipids, and forms 10–15 nm pores, enabling release of mature IL1B, IL18, and CASP7. Related references: PMID:26375003 PMID:26375259 PMID:27418190 PMID:27281216 PMID:29898893 PMID:36227980 PMID:33472215 PMID:37198476
• Mediates non-canonical inflammasome activation in response to cytosolic LPS; also forms pores in mitochondrial membranes, releasing mtDNA, and extracellular N‑terminal fragments directly kill bacteria via lipid‑binding specificity. Related references: PMID:27281216
• Functions as a transcription coactivator in the small intestine after cleavage by CASP3/CASP7; translocates to the nucleus, coactivates STAT1 to induce CIITA and MHC class II, promoting Tr1 cell‑mediated food tolerance.
• Cleaved by papain allergen, leading to plasma membrane pore formation and specific release of IL33, driving a type 2 inflammatory response. Related references: PMID:35794369
• Expressed in suprabasal cells of the esophagus and in the isthmus/neck, pit, and gland of the stomach, indicating preferential expression in differentiating cells.
• Subcellular localization includes cytoplasm, cytosol, inflammasome, cell membrane, secreted, mitochondrion membrane, and nucleus; post‑translational modifications include palmitoylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen spans the Asp275 cleavage site; thus the antibody is expected to selectively recognize the cleaved N‑terminal fragment rather than full‑length GSDMD. Confirm specificity using appropriate positive and negative controls.
• For Western blot, run samples under reducing conditions and probe for a band around 30 kDa (N‑terminal fragment). Validate with lysates from cells undergoing pyroptosis (e.g., LPS+ATP treatment).
• In immunofluorescence/ICC, optimize fixation and permeabilization conditions. The N‑terminal fragment may localize to the plasma membrane or mitochondria in pyroptotic cells.
• For ELISA applications, consider sandwich pairings with a capture antibody targeting a different epitope. Validate performance using recombinant GSDMD proteins or cell‑derived material.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a complete portfolio of validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for pyroptosis and inflammasome research. This anti‑cleaved Gasdermin D rabbit monoclonal antibody exemplifies our capability to deliver high‑specificity raw materials for critical immunology targets. For rare or custom molecular targets, our sourcing team can streamline procurement and technical consultation from concept to clinic.