Anti-Cleaved Caspase-8 (Asp384) Rabbit Monoclonal Antibody for WB, ELISA - Q14790

Core Product Specifications and Parameters

Biological Background: Caspase-8 Function and Localization

• Caspase-8 is a thiol protease that functions as a molecular switch for apoptosis, necroptosis, and pyroptosis, and is essential to prevent tissue damage during development and adulthood. Related references: PMID:23516580 PMID:35338844 PMID:35446120
• As an initiator caspase, it mediates extrinsic apoptosis by forming the death-inducing signaling complex (DISC) upon FAS/CD95 or TNFRSF1A activation, leading to cleavage and activation of effector caspases CASP3, CASP6, CASP7, and others. Related references: PMID:8681376 PMID:8681377 PMID:9184224 PMID:8962078 PMID:9006941 PMID:16916640
• Several isoforms exist: isoform 1 (full-length), isoform 5, and isoform 7 are widely expressed; isoforms lacking the catalytic site act as inhibitors of the caspase cascade. Related references: PMID:12010809
• The protein localizes to the cytoplasm, nucleus, and cell projections such as lamellipodia, and undergoes phosphorylation at Tyr-380 to promote cell migration independently of protease activity. Related references: PMID:18216014 PMID:27109099
• Caspase-8 negatively regulates necroptosis by cleaving RIPK1 at Asp-324, limiting kinase activity and inflammatory responses. Related references: PMID:31827280 PMID:31827281
• It also initiates pyroptosis by cleaving gasdermin-C and gasdermin-D, and participates in innate immunity by inactivating N4BP1 downstream of TLR3/4. Related references: PMID:32929201 PMID:34012073
• Highest expression is observed in peripheral blood leukocytes, spleen, thymus, and liver, with lower levels in brain, testis, and skeletal muscle.
• Keywords include: alternative splicing, zymogen, thiol protease, disease variant, and host-virus interaction.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 371–470 of human Caspase-8 (NP_203519.1), which spans the large subunit region following autocleavage at Asp384; thus, the antibody is specific for the cleaved, activated form.
• For Western blot, the pro-enzyme migrates at approximately 55 kDa, while the cleaved large subunit typically appears around 43–45 kDa. Inclusion of appropriate positive and negative controls (e.g., cells treated with apoptosis inducers) is recommended to verify band identity.
• ELISA optimization may require titration of antibody concentration and careful selection of blocking agents to reduce background; consider using recombinant Caspase-8 protein or peptide as a standard.
• Cross-reactivity with other species has not been determined; validate in the relevant sample system if non-human applications are intended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with comprehensive IVD raw-material procurement, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Our Caspase-8 antibody, targeting the cleaved form, fits seamlessly into apoptosis and cell death assay development. Beyond this product, we provide sourcing solutions for rare targets and technical consulting to streamline your assay development from concept to clinic.