Anti-Cleaved-Caspase 3 p12 Rabbit Monoclonal Antibody for WB, ELISA - P42574

Core Product Specifications and Parameters

Biological Background: Caspase-3 Function and Localization

• Caspase-3, also known as Apopain, CPP32, Yama, or SCA-1, is a thiol protease and major effector caspase executing apoptosis following activation by initiator caspases (CASP8, CASP9, CASP10).
  Related references: PMID:18723680 PMID:20566630 PMID:23650375
• Upon activation, it cleaves numerous substrates including PARP1 at Asp216-Gly217, SREBPs, caspases-6, -7, -9, and IL18, thereby orchestrating cellular disassembly.
  Related references: PMID:10497198 PMID:16374543 PMID:7596430 PMID:37993714 PMID:9334240
• Beyond apoptosis, it contributes to pyroptosis by cleaving gasdermin-E (GSDME), inhibits type I interferon production during virus infection by targeting CGAS, IRF3 and MAVS, and mediates phosphatidylserine exposure via XKR family scramblases.
  Related references: PMID:35338844 PMID:35446120 PMID:30878284 PMID:23845944 PMID:33725486
• Caspase-3 is primarily localized in the cytoplasm and is highly expressed in lung, spleen, heart, liver, and kidney, with moderate levels in brain and skeletal muscle, and highest expression in immune system cells.
• Its activity is regulated by proteolytic cleavage from inactive zymogen to active p12 and p17 subunits, and it undergoes post-translational modifications such as phosphorylation, acetylation, and S-nitrosylation.
• Caspase-3-mediated cleavage of huntingtin is implicated in Huntington's disease pathology, and it also cleaves cell adhesion proteins like DSG2 and JUP to facilitate apoptotic cell detachment.
  Related references: PMID:8696339 PMID:17559062

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the p12 subunit of cleaved caspase-3 (amino acids 171–270); therefore, it is expected to recognize the active cleaved form with high specificity over full-length pro-caspase-3. However, verify cleavage-state specificity in your system.
• For Western blot analysis, consider using apoptosis-induced cell lysates (e.g., staurosporine or etoposide treatment) to detect the cleaved p12 fragment; optimize loading and transfer conditions for this low-molecular-weight protein.
• In ELISA applications, this monoclonal antibody may be suitable as a detection reagent when paired with a capture antibody recognizing a different epitope; ensure proper antibody pair validation in your assay.
• The antibody is validated for human samples; cross-reactivity with other species has not been determined, so confirm reactivity if used with non-human samples.
• As a rabbit monoclonal antibody, it offers reduced lot-to-lot variability; nonetheless, each new lot should be tested in your specific application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research institutions, offering a wide range of IVD raw materials including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Leveraging deep expertise in critical targets like caspase-3, we streamline procurement from concept to clinic, ensuring reliable access to high-quality reagents tailored for apoptosis research and assay development.