Anti-CLDND1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NY35

Core Product Specifications and Parameters

Biological Background: CLDND1 Function and Localization

• CLDND1 (Claudin domain-containing protein 1), also known as Claudin-25 or GENX-3745, is a transmembrane protein encoded by the CLDND1 gene on chromosome 3.
• It functions as a negative regulator of cellular permeability to small molecules, likely contributing to the barrier function of tight junctions.
• The protein localizes to cell-cell junctions, particularly tight junctions, and is integrated into the cell membrane.
• CLDND1 is widely expressed in the adult central nervous system, with high levels in the corpus callosum, caudate nucleus, cerebral cortex, medulla, putamen, spinal cord, substantia nigra, and subthalamic nucleus. Weak expression is also detected in the heart.
• It is subject to alternative splicing, generating protein isoforms, and is characterized as a glycoprotein with a single-pass transmembrane domain.
• Keywords: Alternative splicing, Cell junction, Cell membrane, Glycoprotein, Membrane, Proteomics identification, Reference proteome, Tight junction, Transmembrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 28–138, targeting the N-terminal extracellular region of CLDND1; consider epitope accessibility in native versus denatured conditions.
• For Western blotting, optimize membrane protein extraction and blocking conditions; the observed band may appear at ~29 kDa (slightly higher than the predicted 28.6 kDa due to potential glycosylation).
• Cross-reactivity with mouse and rat indicates conserved epitopes, but validate in your specific sample system and application.
• As a polyclonal antibody, it may offer robust detection in ELISA; titrate the antibody to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-CLDND1 polyclonal antibody supports tight junction research and small molecule permeability studies. We also supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist with rare target raw-material sourcing.