Anti-CLDN7 Polyclonal Antibody for WB, IF-P, ELISA - O95471

Core Product Specifications and Parameters

Biological Background: Claudin-7 Function and Localization

• Claudin-7 is a member of the claudin family of tight junction proteins, encoded by the CLDN7 gene (Gene ID: 1366).
• It plays a major role in tight junction-specific obliteration of the intercellular space, contributing to epithelial barrier integrity.
• The protein localizes primarily to the cell membrane, with enrichment at tight junctions and basolateral membrane domains.
• Claudin-7 undergoes alternative splicing, generating multiple isoforms; isoform 1 is predominantly expressed in most tissues.
• It is a phosphoprotein with four transmembrane helices and a predicted molecular weight of approximately 22-23 kDa.
• CLDN7 is highly expressed in kidney, lung, and prostate. Its expression is frequently downregulated in breast cancers, and loss correlates with higher histological grade.
• Associated UniProt keywords include tight junction, cell junction, membrane, phosphoprotein, alternative splicing, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 29-211, encompassing extracellular and transmembrane regions, and is expected to recognize multiple CLDN7 isoforms.
• For WB, a band at ~22 kDa is anticipated; consider using reducing conditions and validating in relevant cell or tissue lysates.
• IF-P has been validated; for optimal staining, perform antigen retrieval and include appropriate controls for membrane-localized proteins.
• The antibody cross-reacts with mouse and rat; verify reactivity in your specific experimental model.
• For ELISA, titrate the antibody and optimize coating conditions using recombinant CLDN7 or peptide fragments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, polyclonal and monoclonal antibodies, bulk reagents, and rare target sourcing to diagnostic manufacturers and research laboratories. This anti-CLDN7 antibody supports tight junction and cancer biomarker studies, providing a reliable tool for assay development. From concept to clinic, CamelBio bridges your procurement needs with technical expertise and supply chain efficiency.