Anti-CLDN5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O00501

Core Product Specifications and Parameters

Biological Background: Claudin-5 Function and Localization

• Claudin-5, also designated Transmembrane protein deleted in VCFS (TMDVCF), is a member of the claudin family of tight junction proteins.
• It plays a major role in the obliteration of the intercellular space through tight junction formation, critical for establishing epithelial and endothelial barrier function.
• The protein localizes exclusively to cell junctions, specifically tight junctions, and is embedded in the cell membrane.
• Structurally, claudin-5 is a multi-pass transmembrane protein with four transmembrane helices, typical of the claudin family.
• UniProt keywords highlight its involvement in cell junctions, tight junctions, and membrane-related processes.
• The protein is part of the reference proteome for Homo sapiens and has been identified in proteomics studies.

Experimental Guidance and Technical Tips

• The immunogen is designed from an internal sequence (amino acids 119–218) of human CLDN5, covering a region conserved across human, mouse, and rat, which supports the observed cross-reactivity.
• For Western blotting (WB), a band around 23–24 kDa (calculated molecular weight ~23.1 kDa) is expected; consider using appropriate loading controls and standard blocking conditions.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), validate fixation and permeabilization protocols to ensure optimal detection of tight junction-localized CLDN5.
• For ELISA applications, this polyclonal antibody can be utilized as a capture or detection reagent; pairing with a matched monoclonal antibody may improve assay specificity.
• As with any polyclonal antibody, lot-to-lot consistency should be confirmed, and dilution factors should be optimized for each specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive one-stop partner for IVD raw materials, offering a broad portfolio that includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For researchers and diagnostic developers focused on tight junction biology and barrier function, CamelBio provides reliable anti-CLDN5 antibodies and can source rare targets to accelerate your project from concept to clinic. Our integrated technical services and consulting further ensure seamless support for your assay and kit development needs.