Anti-CLDN3 Rabbit Polyclonal Antibody for WB, ELISA - O15551

Core Product Specifications and Parameters

Biological Background: Claudin-3 Function and Localization

• Claudin-3, also known as CPE-receptor 2 (CPE-R2) and RVP1, is a member of the claudin family of tight junction proteins.
• It functions as a barrier-forming claudin, playing a critical role in tight junction-specific obliteration of the intercellular space.
• Claudin-3 mediates calcium-independent cell adhesion, contributing to the sealing of the paracellular pathway.
• The protein is localized at cell junctions, specifically tight junctions, and is an integral membrane protein with four transmembrane helices.
• Claudin-3 is subject to phosphorylation, which may regulate its assembly and function at tight junctions.
• Genetic alterations involving CLDN3 are associated with Williams-Beuren syndrome, a developmental disorder.
• It has a molecular weight of approximately 23 kDa.

Experimental Guidance and Technical Tips

• When using this antibody for Western blot, consider using whole-cell lysates from human, mouse, or rat epithelial cell lines, and expect a band at approximately 23 kDa.
• For ELISA, titrate the antibody to determine optimal working concentration, and include appropriate controls.
• Since the immunogen spans the C-terminal region (amino acids 141–220), the antibody may recognize both full-length and potential C-terminal fragments; validate in the relevant sample system.
• Given the cross-reactivity with mouse and rat, sequence alignment of the immunogen region across these species is recommended to assess epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for diagnostic assay development. For tight junction and cell adhesion research, we can supply CLDN3 antibodies and related reagents to support your workflow. Leverage our one-stop services for rare target sourcing and technical consultation to accelerate your project from concept to clinic.