Anti-CLDN17 Polyclonal Antibody for WB, IHC-P, ELISA - P56750

Core Product Specifications and Parameters

Biological Background: Claudin-17 Function and Localization

• Claudin-17 is a transmembrane tight junction protein belonging to the claudin family, which plays a critical role in paracellular permeability and ion selectivity.
• It functions as a channel-forming tight junction protein with selectivity for anions, including chloride and hydrogencarbonate, and permits solutes smaller than 9 Angstrom in diameter; water permeability is not affected.
• In the kidney, claudin-17 contributes to paracellular reabsorption of filtered anions in the proximal tubule, helping to maintain electrolyte balance.
• It is localized at cell junctions, specifically the tight junction, and is also detected at the basolateral cell membrane, indicating a role in cell polarity and barrier function.
• Tissue expression is predominantly in the kidney: the proximal tubule, Henle's loop, and a subset of distal convoluted tubules, but it is absent from the collecting duct (at protein level).
• The protein shares structural features with other claudins, including four transmembrane helices, and is implicated in ion channel and transport activities as reflected by its keywords (Chloride, Chloride channel, Ion channel, Ion transport).
• Molecular weight is approximately 24.6 kDa (calculated), and it contains key motifs for membrane integration and junctional assembly.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 100 to C-terminus) of human CLDN17, which may confer isoform specificity; consider verifying sequence homology with mouse and rat orthologs due to reported cross-reactivity.
• For Western blotting (WB), a band around 25 kDa is expected; validate in appropriate positive control lysates (e.g., kidney tissue or cell lines expressing CLDN17).
• For immunohistochemistry (IHC-P), antigen retrieval optimization may be necessary, and staining is anticipated in kidney proximal tubules; include relevant controls to confirm specificity.
• In ELISA applications, the peptide immunogen can serve as a positive control; assay conditions should be optimized empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop provider of IVD raw materials, supporting diagnostic manufacturers and researchers with high-quality antibodies, validated antibody pairs, bulk ancillary reagents, and sourcing of rare targets. As a B2B partner for tight junction and renal physiology research, CamelBio streamlines procurement of optimized polyclonal and monoclonal antibodies for routine and specialized applications. For your anion transport and kidney disease studies, count on CamelBio’s expertise to accelerate your assay development from concept to clinic.