Anti-CLDN11 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75508

Core Product Specifications and Parameters

Biological Background: Claudin-11 Function and Localization

• CLDN11 encodes Claudin-11 (OSP/OTM), a 22 kDa integral membrane protein of the claudin family.
• Claudin-11 functions as a crucial tight junction component, mediating calcium-independent intercellular adhesion to seal the paracellular space.
• Subcellular localization is predominantly at tight junctions of the cell membrane, where it contributes to barrier formation.
• Claudin-11 is characterized by four transmembrane domains, facilitating its role in cell-cell contact.
• The protein is subject to phosphorylation, suggesting dynamic regulation of tight junction assembly and function.
• Mutations in CLDN11 are linked to leukodystrophy, underscoring its role in central nervous system myelin integrity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-100); consider epitope accessibility in your experimental design.
• For Western blotting, expect a band near 22 kDa; optimize membrane protein extraction protocols and use proper loading controls.
• In IHC-P, antigen retrieval may be necessary; validate staining in tissues known to express CLDN11, such as brain or testis.
• When performing ELISA, coat with the immunizing peptide or recombinant CLDN11 protein to confirm antibody specificity.
• Cross-reactivity with mouse and rat has been reported; verify reactivity in your sample system before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with reliable access to premium IVD raw materials. This anti-CLDN11 antibody supports neuroscience and barrier function studies and can be combined with validated antibody pairs, ancillary reagents, or custom monoclonal development services. Streamline your assay development with our efficient, end-to-end procurement platform.