Anti-CLDN11 Rabbit Polyclonal Antibody for WB, ELISA - O75508

Core Product Specifications and Parameters

Biological Background: Claudin-11 Function and Localization

• Claudin-11, also known as oligodendrocyte-specific protein (OSP), is a member of the claudin family of tight junction proteins.
• It plays a major role in tight junction-specific obliteration of the intercellular space through calcium-independent cell-adhesion activity, contributing to barrier formation.
• Claudin-11 is primarily localized at cell junctions, specifically tight junctions, and is also found on the cell membrane.
• The protein is a multi-pass transmembrane protein, as indicated by its transmembrane helix keywords; it undergoes phosphorylation (phosphoprotein).
• Mutations in the CLDN11 gene are associated with leukodystrophy, highlighting its importance in myelin integrity and central nervous system function.
• UniProt annotates it as a reference proteome protein, with a molecular weight of approximately 22 kDa.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 26-207, covering most of the protein including extracellular loops; consider this when evaluating potential cross-reactivity with other claudin family members.
• For Western blot, a band around 22 kDa is expected; validate detection in relevant sample systems such as brain tissue lysates or cell lines expressing CLDN11.
• In ELISA applications, confirm the antibody’s performance using recombinant CLDN11 protein or relevant peptide controls.
• Sample preparation for membrane proteins may require specific lysis buffers to ensure proper solubilization; consider using detergents like SDS or RIPA buffer.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, supporting tight junction research and leukodystrophy studies. Whether you need rare target antibodies or reliable supply chains for diagnostic development, CamelBio bridges your research from concept to clinic with expert sourcing and technical services.