Anti-CLDN10 Rabbit Polyclonal Antibody for WB, ELISA - P78369

Core Product Specifications and Parameters

Biological Background: Claudin-10 Function and Localization

• Claudin-10 is a tight junction protein encoded by the CLDN10 gene; alternative splicing produces multiple isoforms, and it belongs to the claudin family.
• It functions as a paracellular ion-selective channel former, polymerizing in tight junction strands to regulate epithelial permeability.
• Claudin-10 forms cation-selective paracellular channels, controlling paracellular sodium permeability in sweat glands and the kidney's thick ascending limb, essential for sweat production and renal function. Related references: PMID:19383724, PMID:28686597, PMID:28771254
• It also forms anion-selective channels, facilitating selective chloride over bicarbonate permeability in renal proximal tubules, required for chloride reabsorption and salt homeostasis.
• Tissue expression is prominent in kidney (specifically the thick ascending limb of Henle's loop), eccrine sweat glands, and all layers of the epidermis. Related references: PMID:28686597, PMID:28771254
• The protein localizes to tight junctions and the cell membrane.
• Keywords include alternative splicing, disease variant, ichthyosis, and ion transport, highlighting its role in skin disorders and electrolyte balance.
• The molecular weight is approximately 24.5 kDa (UniProt: 24488 Da).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100); for Western blot, consider using reducing and denaturing conditions to ensure complete linearization of the target protein.
• Validate antibody specificity using positive controls such as human kidney or sweat gland tissue lysates; cross-reactivity with rat suggests potential use in rat models, but experimental confirmation is recommended.
• For ELISA, optimize the peptide coating concentration and blocking conditions to achieve an adequate signal-to-noise ratio.
• Store the antibody as recommended and avoid repeated freeze-thaw cycles to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with comprehensive access to IVD raw materials, from validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents. For tight junction and renal physiology research involving Claudin-10, we offer one-stop sourcing support, including custom antibody development and rare target procurement. Partner with us to streamline your development from concept to clinic.