Anti-CLCNKA Rabbit Polyclonal Antibody for WB, ELISA - P51800

Core Product Specifications and Parameters

Biological Background: CLCNKA Function and Localization

• CLCNKA encodes the chloride channel protein ClC-Ka (also known as ClC-K1), a member of the ClC family of anion-selective channels.
• Functions as a homodimeric channel where each subunit forms an independent ion conduction pathway, permeable to small monovalent anions with selectivity for chloride > bromide > nitrate > iodide. Related references: PMID:11734858 PMID:12111250
• Channel activity is controlled by two types of gates: fast gates that regulate each subunit independently and a slow common gate that simultaneously opens and shuts off both subunits. Related references: PMID:11734858 PMID:12111250 PMID:18310267
• Requires assembly with the regulatory subunit BSND/Barttin for proper sorting to the basolateral plasma membrane and functional switching to the ion-conducting state. Related references: PMID:11734858 PMID:12111250 PMID:18310267
• Mediates transepithelial chloride transport in the thin ascending limb of Henle's loop, contributing to the generation of a hypertonic medullary interstitium essential for urine concentration. Related references: PMID:15044642
• Conducts chloride currents in the stria vascularis of the inner ear to establish the endocochlear potential, necessary for normal hearing. Related references: PMID:15044642 PMID:18310267 PMID:19646679
• Subcellularly localized to the basolateral cell membrane; associated diseases include Bartter syndrome and deafness (from Keywords).

Experimental Guidance and Technical Tips

• The immunogen was designed using a C-terminal fragment (amino acids 470-644), so the antibody may preferentially detect full-length CLCNKA; confirm specificity with appropriate controls.
• For Western blot, expect a band around 75 kDa in human and mouse tissue lysates; consider using positive controls such as kidney or inner ear samples.
• Include negative controls (e.g., CLCNKA knockout cell lines) to validate band identity.
• In ELISA, titrate the antibody using recombinant CLCNKA protein to determine optimal working concentration.

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