Anti-CLCN7 Rabbit Polyclonal Antibody for WB and ELISA - P51798

Core Product Specifications and Parameters

Biological Background: CLCN7 Function and Localization

• CLCN7 encodes the H(+)/Cl(-) exchange transporter 7 (ClC-7), a member of the CLC family of chloride channels and transporters.
• It mediates slowly voltage-gated chloride/proton antiport, contributing to lysosomal lumen acidification and pH homeostasis. Related references: PMID:18449189 PMID:21527911 PMID:31155284
• Localizes predominantly to the lysosome membrane.
• Expressed in brain and kidney tissues.
• Mutations in CLCN7 are associated with osteopetrosis, a bone density disorder.
• Contains conserved CBS domains and an ATP-binding site, suggesting regulation by nucleotide binding.
• Exhibits alternative splicing and phosphorylation.
• The antiporter mechanism depends on conserved gating glutamate residues.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 626–805 of human CLCN7, a region with high sequence homology across human, mouse, and rat, consistent with the observed cross-reactivity.
• For Western blot, a band at ~89 kDa is expected; optimize antibody dilution and detection conditions for your samples.
• This polyclonal antibody is suitable for both WB and ELISA applications; consider pairing with a monoclonal antibody for sandwich ELISA if needed.
• Validate performance in your specific experimental system, especially when studying endogenous versus overexpressed protein levels.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio stands as a trusted one-stop partner for IVD raw-material sourcing, from concept to clinic. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized raw materials for rare targets. For CLCN7 and related ion transport research, CamelBio streamlines procurement and supports your diagnostic development needs.